FocalPlane is a community site for anyone with an interest in microscopy.
This #TechnicalTuesday we’re reading the #preLight from Girish Kale highlighting the beautiful #preprint on embryo mechanics from Sacha Ichbiah, Hervé Turlier, & team.
@preLights https://prelights.biologists.com/highlights/embryo-mechanics-cartography-inference-of-3d-force-atlases-from-fluorescence-microscopy/
The deadline for Workshop proposals with @Co_Biologists is fast approaching. We’re looking for proposals that address cutting-edge science on emerging themes.
Deadline: 30 June 2023
Check out our post to find out more: https://focalplane.biologists.com/2023/06/12/the-company-of-biologists-workshops-2025/
This #EMmonday we are focussing on SBF-SEM with a case study from Federica Mangione. Federica and colleagues used SBF-SEM to dissect the cellular assembly of tactile organ of Drosophila, identifying a new cell type, the F-Cell.
https://focalplane.biologists.com/2023/05/19/inputs-and-outputs-of-vem-in-a-sensory-system/
For more from the volume EM community: https://focalplane.biologists.com/category/volume-em/
We’re celebrating #FluorescenceFriday by highlighting the #OpenAcess @BiologyOpen study from Siyu Yang, Mingzhe Cao and colleagues. They use #zebrafish to investigate retinal development and function and find that loss of flrt2 leads to microphthalmia.
📢 New post on FocalPlane
Next up in our rotation of preprints topics are new tools and techniques in imaging. The list this week includes advances in hardware, sample preparation, probes and accessible imaging systems. Let us know if we have missed any recent preprints from your reading list. https://focalplane.biologists.com/2023/06/16/microscopy-preprints-new-tool-and-techniques-in-imaging-5/
🌟 Deadline incoming for our 2023 Image competition!
Get your images to us by Monday 19 June for your chance to see your image on the cover of @J_Cell_Sci and win £100. Thanks to everyone that has already submitted image, we can’t wait to share them!
https://focalplane.biologists.com/2023/05/15/focalplane-image-competition-2023/
This is a nice summary on @focalplane_jcs of visualisation tools for 3D light #microscopy data.
https://focalplane.biologists.com/2023/06/14/community-collective-viewing-z-stacks-in-3d/
My go to is #ClearVolume or #ChimeraX (we steered clear of Imaris). Very interested to try out 3Dscript.
📢 New post on FocalPlane
In our ‘Community collective’ posts we aim to give a permanent (and searchable) home for those nuggets of advice collected from the community. Our first post focusses on 3D viewers after a request for software recommendations from @engineeringjoy
https://focalplane.biologists.com/2023/06/14/community-collective-viewing-z-stacks-in-3d/
If you have requested (or seen) any microscopy advice that you would like to see be given a home on FocalPlane, comment here or contact us at focalplane@biologists.com
Our aMAZ(E)ing #MidweekMovie comes from Connie Shen, Judith Mandl, Nienke Vrisekoop and colleagues. They discover that neutrophils with segmented nuclei have a speed advantage when migrating through confinement spaces
📖 @J_Cell_Sci: https://journals.biologists.com/jcs/article/136/11/jcs260768/316606/Nuclear-segmentation-facilitates-neutrophil
📢 New post on FocalPlane
We are excited that @KuglerElisabeth, Director at Zeeks – Art for Geeks, shares her top tips on moving from academia to industry in a post FocalPlane. Elisabeth uses her personal journey to give advice that will be useful for any career path.
https://focalplane.biologists.com/2023/06/12/top-5-tips-for-the-shift-from-academia-to-industry/
This #TechnicalTuesday we are highlighting a FocalPlane post from Mara Lampert on tracking in napari. Mara describes the different tracking options, guides the user through the process & calls for feedback on the tools.
https://focalplane.biologists.com/2023/06/01/tracking-in-napari/
You can find Mara’s series, ‘Bio-image Analysis with Napari here: https://focalplane.biologists.com/2023/06/01/tracking-in-napari/
Interested in building genetic tools to study development and regeneration? We're looking for research engineer to spearhead the development of transgenic tools in the emerging crustacean model Parhyale
Did you know…Journal of Cell Science shows a strong ethical approach to publishing best-practice, enabling us to publish research that is trusted. With our plagiarism and image manipulation checks, transparent peer-review process, copyediting by experienced scientific editors, and simple solutions for data sharing and access, we make sure that you – as readers and authors – can focus on the science.
This #EMmonday we are looking at these beautiful L. mexicana axoneme cross-sections from Chandra Ramakrishnan, Cécile Fort, Linda Kohl, Richard Wheeler, Robert Sinden and colleagues. They examined the role of Rsp9 in axoneme assembly.
#OpenAccess
Belated welcome to new postdoc Lizzie Courthold who joined us this week. You can meet all of us here:
In their #OpenAccess Research Article, Chandra Ramakrishnan, Cécile Fort, Linda Kohl, Richard Wheeler, Robert Sinden, et al. find that RSP9 is required for axoneme assembly in malaria, but not trypanosomatid, parasites.
This article is available under our Read & Publish Open Access initiative.
Researchers can find out about the wide range of benefits and view a list of participating institutions at https://www.biologists.com/library-hub/read-publish/researchers/
Happy #FluorescenceFriday! Eunice Silva, Ana Venda and Catarina Homem find that serine hydroxymethyl transferase is required for optic lobe neuroepithelia development in Drosophila.
📢New post on FocalPlane
You can now view the talk from
Bruno Combettes (Andor), presented at #celldyn2023. Bruno talked about the real time SRRF (SRRF-Stream) technology from Andor.
https://focalplane.biologists.com/2023/06/07/celldyn2023-andor-srrf-stream/
Brenda Delfin, a researcher at Universidad Peruana Cayetano Heredia, in Lima, Peru, discusses her research career with Mariana De Niz, in the latest ‘Latin American Microscopists’ interview. Brenda discuss the importance of mentorship and education, as well as the challenges and future hopes for science in Peru.
https://focalplane.biologists.com/2023/06/06/an-interview-with-brenda-delfin/
#microscopy #microscopy #LatamBioimaging
Our #MidweekMovie shows actin dynamics in a #zebrafish embryo from Haruka Oda, Hiroshi Kimura and colleagues. They find that filamentous actin accumulates in the nucleus of early embryos and forms patches associating with condensing chromosomes during prophase.
📖 @BiologyOpen https://journals.biologists.com/bio/article/12/5/bio059783/310414/Actin-filaments-accumulated-in-the-nucleus-remain
