Lmst

Had to revisit our paper from 2018 for a future project…boy I love the immunofluorescence microscopy we did on the human placenta.
#FluorescenceFriday #Science #Placenta #ReproSci
https://bmcdevbiol.biomedcentral.com/articles/10.1186/s12861-018-0178-0

terbium something (idk?) #fluorescencefriday

round bottom flask on a hotplate with a suspension of grey product in it

same flask under uv exhibiting fluorescence emitting green light

Linda semana, sumergido en el mundo microscópico…
🔬🦠

#mitochondria
#fluorescencefriday

Imagen de microscopía de fluorescencia que muestra células cultivadas. Los núcleos celulares están teñidos de azul y se observan como estructuras ovaladas prominentes. Las mitocondrias están teñidas de verde y se distribuyen alrededor del núcleo con una disposición densa y filamentosa en el citoplasma. El fondo es negro, resaltando el contraste de los colores fluorescentes

#FluorescenceFriday

Front yard😊
Not really fluorescence, but like fluorescence 😉

#Flower #Fluorescence #Plant #Yard #Photography

Purple hyacinths, yellow mini daffodils, and white Glory of the Snow are blooming.

For #fluorescencefriday I present: stoma falling into the abyss, on a dying flower. The other tech gave me beautiful purple flowers for my birthday and I just had to take a look. In microscopy I’m always interested how things tear/break/rip and otherwise fall apart... #microscopy #sciart

#FluorescenceFriday

Some nice colors of Dragon fruit 😊😋
This reminds me that I should plan some fluorescence microscopy experiments 🤔

#DragonFruit #Fruits #Photography #Fluorescence #Science #Experiment

One half of the Dragon fruit is cut in dice shape, and the other half is not.

New publication from Kwanghun Chung lab at MIT improves on their SWITCH approach and eFLASH stochastic electrotransport to improve antibody labeling by gradually shifting the microenvironment. Looks interesting, I wonder how well it can be adapted to other labeling protocols.

Uniform volumetric single-cell processing for organ-scale molecular phenotyping
Yun et al., Nature Biotechnology 2025
https://doi.org/10.1038/s41587-024-02533-4

#neuroscience #tissueclearing #lightsheet #microscopy #fluorescenceFriday

Fig. 4: Holistic comparison of transgenic and immunolabeling-based cell type labeling. a–c, 3D dataset from a PV-Cre and loxP-tdTomato dual transgenic mouse hemisphere stained with anti-PV antibody. a, Representative optical section. b, Magnified images of a. c, A percentage plot for tdTomato-only (red), anti-PV-only (green) and tdTomato and anti-PV co-positive cells (yellow) among all the labeled cells in individual representative brain regions. d–h, 3D dataset of a ChATBAC-eGFP mouse brain stained with anti-ChAT antibody. d, Whole volume rendering. e, Magnified images of d. f, A percentage plot for EGFP-only (green), anti-ChAT-only (red) and EGFP and anti-ChAT co-positive cells (yellow) among all the labeled cells in individual representative brain regions. g, Magnified view of d. h, Zoom-in view of g. Scale bars, 2 mm (cyan) and 200 μm (white). 5N, motor nucleus of trigeminal; A1, primary auditory cortex; AC, anterior cingulate cortex; BLAa, basolateral amygdala, anterior part; BLAp, basolateral amygdala, posterior part; CA1, hippocampal CA1; CA3, hippocampal CA3; CeA, central amygdala; DG, dentate gyrus; dNAmb, nucleus ambiguus, dorsal part; Ecto, ectorhinal cortex; LA, lateral amygdala; lEnto, lateral entorhinal cortex; mo, dentate gyrus, molecular layer; po, dentate gyrus, polymorph layer; Piri, piriform cortex; PPA, posterior parietal association cortex; RSA, retrosplenial cortex; sg, dentate gyrus, granule cell layer; V1, primary visual cortex;

I missed #FluorescenceFriday but here's an image for one from the manuscripts on the nearly-finished pile. We've found a genetic marker for the deep CA1 pyramidal cells. Magenta is calbindin (IHC), cyan is the other cell type (AAV-TdTom). Less than 5% overlap. Coming to BioRxiv by Easter, hopefully! #Neuroscience

Image of different subsets of CA1 pyramial cells, with calbindin-positive cells in magenta and the other cell type in cyan (IHC for calbindin, AAV-driven fluorophore expression for the other).

Night sky in the thalamus. Or is it cities from high above? Fluorescent microscopy is my fave so I’m super happy to be on the confocal this week. #fluorescencefriday #neurons #sciart

You can check it out here: https://doi.org/10.1016/j.jid.2024.10.595

#fluorescencefriday every day...

We are especially grateful to CMN patient advocacy groups, MELCAYA, AFM-Téléthon, and the Institut Marseille Maladies Rares (MarMaRa), for their support.

Bon bout d'an à tous ! (Happy nearly-New Year to all!)

-fin-

My contribution to #FluorescenceFriday is this mysterious hot pink plant debris with algae on it, so many little shapes and details! Along with the trails of flagellates (orange) and cladocerans (blue-green). #SciArt #chronophotography #microscopy #daphnia

Is #FluorescenceFriday still a thing? I recently made my very first youtube video- in it I compare my triple bandpass fluo filter to a UV longpass filter imaging autofluorescence of plankton: youtu.be/n2rCXnJ50Y8?... Love having a fluo setup! Below, decomposing algae in both filters. #SciArt

@MFM_BIC@synapse.cafe @MFM_BIC

ICYMI you can follow tags in here:

#FluorescenceFriday
#NeuroImaging
#CellBiology

On #FluorescenceFriday, we’re highlighting this beautiful SIM image from @dgaboriau These human fibroblasts are labelled to visualise mitochondria (Tom20) in yellow, alpha-tubulin in pink and actin in grey.
https://focalplane.biologists.com/2024/10/11/featured-image-with-david-gaboriau/

#FluorescenceFriday

Some nice telomere signals, I think experiments are working

#Telomere #Fluorescence #CellBiology #MolecularBiology #GraduateSchool

Fluorescent images of cells with green dots where telomeres are located. The background is black.

for #FluorescenceFriday, here's a video of two-photon imaging 🔬 from mouse visual cortical neurons at 0.5x speed in response to many natural images #neuroscience (paper here: https://www.biorxiv.org/content/10.1101/2024.06.30.601394v1)

Happy #fluorescenceFriday, let’s see those gorgeous images!

#FluorescenceFriday

It’s #FluorescenceFriday & we’re highlighting Shivangi Verma’s (@shivangiv10) image of a #zebrafish larval brain. The larva expressing GCaMP6f was embedded in agarose & imaged using a Zeiss Lightsheet microscope. Find out more about Shivangi's research:
https://focalplane.biologists.com/2024/08/30/featured-image-with-shivangi-verma/

Happy #FluorescenceFriday

Some actin action always - amazes me (and gets me safely into the weekend) 😜

Timelapse imaging on Zeiss880 of HEK cells expressing Lifeact-mEGFP
LUT is Cyan Hot (quite like that one)

#FluorescenceFriday
(is this a thing on #Mastodon - if not let's make it one!)

Nuclei labelled with EGFP-H2B.
MIP of a small z-stack at two frames per minute.

Loads of action, movement and cell divisions, in the developing zebra fish hindbrain 🐟🧠

#FluorescenceFriday

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