#NeuroMethods I am looking for a UK supplier of thin metal cannulas.
Specifically, I would like a relatively small one (27Ga, thin wall, this one: https://componentsupplycompany.com/hypodermic-tubing/304ss-thin-wall/htx-27t-30/
This one was recommended to me
https://www.coopersneedleworks.com/
but they cannot make 27 Ga :(
Any other suggestions? š
#HyperDrive building
#NeuroMethods question for those doing rodent surgeries:
Has anyone tried out this Stoelting #Stereotax ? What did you think?
https://stoeltingco.com/Neuroscience/Digital-Rat-Stereotaxic-Instrument~9637
I've said it before but the #NeuroMethods slack is really useful for all #Neuroscience methods questions. Feel free to join at https://neuromethods.slack.com/ with your institutional email, or DM me if you can't join that way!
Hi all, the recurrent question of backing up (or not) #NeuroMethods slack is there again.
Lots of people are suggesting backing up the contents onto some #Google product, whether gmail or google docs. I know that gmail is not private (google analyses the contents of the mails) but is Google document private?
Otherwise is there an alternative to google docs that would actually be private? I imagine if it is private then it will not be free..
(Neuromethods is a discussion platform for when you have a problem or question about a #Neuroscience technique. It's on the free #Slack version so the history past 90 days is lost. See the previous time I have asked about this: https://neuromatch.social/@elduvelle/112766589207831557)
#NeuroMethods question:
What do you all (experimentalists or computational people) prefer as a format to store and share #Electrophysiology data? Either for Tetrodes or Probes? And why? š
Is NWB (Neuroscience Without Borders) the gold standard?
#VTE detection: what's the best algorithm out there to detect Rat VTEs from 2D head position data (no HD data)?
By "best" I mean least effortful for the user...
I know that @adredish has the D-phil method (explained in Interactions between deliberation and delay-discounting in rats #RedishLab which is used quite a lot.
But also just found out about a different method from @jessetm explained in A Machine Learning Approach for Detecting Vicarious Trial and Error Behaviors #MizumoriLab - Jesse, I haven't checked the details yet but as its creator, would you recommend it? Would you have code for it somewhere? I couldn't see a repo link in the paper but maybe I missed it. š
Edit: Jeff Stott added this one to the list, which uses a "Curvature" measure (also from #RedishLab): A functional difference in information processing between orbitofrontal cortex and ventral striatum during decision-making behaviour
Any other ones out there?
#Neuroscience #NeuroMethods #SpatialCognition
UK hardware question: where can you buy small metal cannulas, as well as polyimide and polymicro tubing? (Things used for #Hyperdrives)
I know the places for the US but have no idea for UK (where I am now)
Edit: by small I mean something in the range of 100-300 microns diameter
I don't think it would be possible to record a single neuron on multiple tetrodes. What is the separation between your tetrodes?
#NeuroMethods #Ephys #SpikeSorting #Tetrodes
If you do need cross-correlation code, there is mex'd cross-correlation code in MClust.
#NeuroMethods #Ephys what is the fastest way to detect potential duplicate neurons after spike-sorting?
Say youāve recorded from multiple neurons that might be detected by multiple tetrodes, you do #SpikeSorting per tetrode, and want to detect those duplicates afterwards?
I was thinking cross-correlation across all cell pairs. But my implementation is really slow. If anyone already has code for this (Matlab or Python) Iād be happy to steal it š¬
Seen on the #NeuroMethods slack:
Any suggestions for algorithms to track pupil in mice? (Not #DeepLabCut )
The #NeuroMethods slack is an amazing resource for all #Neuroscience technical questions. However itās using a free slack version so all older messages are hidden, and with new #Slack policies theyāll soon be deleted.
One solution would be to find one or several āsponsorsā who would help move the slack to a paid version. Any ideas or suggestions about this?
(An older article on the Neuromethods slack: https://www.bna.org.uk/mediacentre/news/neuromethods-slack-group-a-new-platform-for-troubleshooting-methods-in-neuroscience/
The link to sign-in: https://neuromethods.slack.com/
Ask me here or in DM and Iāll invite you if you canāt sign in with your work email!)
@susanleemburg oh I saw your post on #NeuroMethods. Hope you find your answer!
What do #Electrophysiologists who record raw data from many channels (#NeuroPixels, #Hyperdrivesā¦) do for data backup and storage? I just bought 2 x 4Tb external drives and it looks like they wonāt be enough (at all). Is there a cheap and reliable AND huge storage way to do this?
Whatās your best guide on extracellular recordings #waveform shapes to know if youāre recording from soma, axon, dendrite etc.? (Most interested about #Hippocampus of course)
#Ephys #Electrophysiology #NeuroMethods
#NeuroMethods question:
Has anyone found a good, reliable, automatized way to deliver small liquid reward amounts for rodent experiments?
Weāve been using a pressurized air system with solenoid valves (from McMaster) but they are not very reliable and the quantity delivered fluctuates with time and depends on the actual valveā¦
We were thinking of testing out the Open Neuroscience / #LaubachLab syringe pump but someone just mentioned they are not that reliable for small volumes (1-10 microliters). Anyone else has feedback on them?
Anyone doing liquid reward delivery for rodent who has found the Valve Graal??
#Neuroscience #BehaviouralNeuroscience
What are some metals that rats not only cannot chew, but also do not like to chew on?
Trying to find the best material for a kind of nosepokeā¦
#NeuroMethods #NeuroRats
#NeuroMethods question:
What is the smallest optimal distance between independent #Tetrodes in dorsal CA1 (so that you donāt record from the same neuron in adjacent tetrodes and also movement of one doesnāt influence the movement of others)?
Say, using 12.7 Ī¼m diameter wire?
Do I know anyone using the #NeuroMouse version of the #ShuttleDrive?
(Small, 32 (or 16?) tetrodes version)
If so can you tell me what you think in terms of stability, recording quality etc?
https://open-ephys.org/shuttledrive
@biogeo thanks for the details - very interesting!
The neurologger I know of is the #SpikeGadgets one and it records 128ch of high res LFP (unfiltered) so you can get spikes from that using any kind of filtering + spike detection. But it is not compatible with MRI. They also have one for Neuropixels probes but I havenāt tried it.
You might have more luck asking your question on the #NeuroMethods slack (https://neuromethods.slack.com/) - let me know if you need an invite!
Iāve started making tutorials for different #Tetrode #Electrophysiology steps here
The next ones are probably going to be Tetrode-making and drive-building.
Is there anything else that youād like to see there?
#NeuroMethods
