#ampliconsequencing

Teresita Porter 🙋🏻‍♀️DNAdataPhile@ecoevo.social
2023-06-27

"rarefaction was actually the most robust approach for controlling for uneven sequencing effort across samples. Rarefaction limits the rate of falsely detecting and rejecting differences between treatment groups. Far from being "inadmissible", rarefaction is a valuable tool for analyzing microbiome sequence data."

Waste not, want not: Revisiting the analysis that called into question the practice of rarefaction

#metabarcoding #rarefaction #AmpliconSequencing

doi.org/10.1101/2023.06.23.546

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