The SAMPREP workshop at the 2023 ACA Annual Meeting in Baltimore was designed to address the need to consoiidate cross-disciplinary best practices for sample preparation into a single forum @Penn #SamplePreparation #StructuralBiology #CryoEM https://doi.org/10.1107/S2053230X25007976
#cryoEM
Cryo‑EM is hitting near‑atomic resolution and even capturing molecular motion over time. It’s wild to see huge complexes rendered with such clarity—like the supplementary videos in that recent paper by Muyuan Chen. The leap from nanometer to atomic scale in a decade feels like a paradigm shift for mechanism insight and rational drug design. What practical workflows are you adopting around these maps?
Muyuan Chen has turned structural biology into an immersive experience with his new video game Meowtabolism, now available on Steam. Explore the interior of a cell on four paws in an environment built from experimental data and real protein structures.
Try the demo here: https://store.steampowered.com/app/4045010/Meowtabolism__Examining_Feline_Behavior_within_Unicellular_Algae/
Muyuan is seeking feedback: https://steamcommunity.com/app/4045010
Or you can email him directly.
🤖 What happens when we train AI to see through the noise of cryo-EM imaging?
🔗 A labeled dataset for AI-based cryo-EM map enhancement. Computational and Structural Biotechnology Journal, DOI: https://doi.org/10.1016/j.csbj.2025.06.041
📚 CSBJ: https://www.csbj.org/
#AI #CryoEM #StructuralBiology #DeepLearning #OpenScience #ProteinStructures #MolecularBiology #Macromolecules #BigData
Capturing enzymes in motion
The structure and dynamics of the ACE enzyme revealed by #CryoEM and molecular simulations.
https://elifesciences.org/articles/108727?utm_source=mastodon&utm_medium=social&utm_campaign=organic_insights
Forgetting the gain reference file is why you don't finalize the deposition of a #cryoEM dataset to EMPIAR on a Friday evening... 🤦♂️
Good that I have two weeks to fix this.
CryoSPARC now has several fancy algorithms to sort heterogeneity. But the more #cryoEM datasets I process, the more I believe that 3D classification (with no alignment) and local refinement are all we need. Combined with some understanding of the particle under study and carefully crafted masks, they are applicable to (and in fact solve) so many cases.
Today's Friday project is revisiting a dataset that has been sitting around since the first pass analysis had mostly convinced us that it didn't contain the complex we were after. Keyword "mostly". Revisiting this dataset now by 3D classification under new assumptions (occupancy of the component of the complex we are looking for may well be much lower than expected from the biochemistry). Don't want to jinx it until I see a refined map, but it starts looking like I found that little protein! 🤩
https://www.biorxiv.org/content/10.1101/2025.09.08.674935v1
Cool usage of ab initio by Oli Clarke's lab #cryoEM
Postdoctoral Associate in Membrane Protein Structural Biology
Vecchio Lab at University at Buffalo
#postdoc in vecchio lab at university at buffalo department of #structuralbiology studying #membraneprotein #structureandfunction
See the full job description on jobRxiv: https://jobrxiv.org/job/university-at-buffalo-27778-postdoctoral-associate/
#biophysics #cryoEM #membraneproteins #proteinproteininter...
https://jobrxiv.org/job/university-at-buffalo-27778-postdoctoral-associate/?fsp_sid=2412
Erice Structural Biology Course 2026 (29 May–6 Jun): apply! Train in Cryo-EM, Cryo-ET & ML to study macromolecular assemblies in cells. Hands-on + theory for next-gen structural cell biology.
🔗 erice2026.azuleon.org/application....
#CryoEM #CryoET #MachineLearning #StructuralBiology #CellBiology
International School of Crysta...
PhD student in cryo-ET of membrane trafficking at MPI-NAT in Göttingen, DE
Max-Planck-Institute for Multidisciplinary Science
Join our #CryoEM lab at MPI-NAT in Göttingen for a PhD in membrane trafficking
See the full job description on jobRxiv: https://jobrxiv.org/job/max-planck-institute-for-multidisciplinary-science-2777...
https://jobrxiv.org/job/max-planck-institute-for-multidisciplinary-science-27778-phd-student-in-cryo-et-of-membrane-trafficking-at-mpi-nat-in-gottingen-de/?fsp_sid=2090
OMG the newest Coot is on Flathub! 🤩
I wish I had known earlier.
Postdoctoral Associate in Membrane Protein Structural Biology
Vecchio Lab at University at Buffalo
#postdoc in vecchio lab at university at buffalo department of #structuralbiology studying #membraneprotein #structureandfunction
See the full job description on jobRxiv: https://jobrxiv.org/job/university-at-buffalo-27778-postdoctoral-associate/
#biophysics #cryoEM #membraneproteins #proteinproteininter...
https://jobrxiv.org/job/university-at-buffalo-27778-postdoctoral-associate/?fsp_sid=1950
🌀 Could a protein that twists into filaments reveal how cells keep themselves in balance?
🔗 Cryo-EM structure of AAA + ATPase thorase reveals novel helical filament formation. Computational and Structural Biotechnology Journal, DOI: https://doi.org/10.1016/j.csbj.2025.05.018
📚 CSBJ: https://www.csbj.org/
#StructuralBiology #CryoEM #Thorase #MolecularBiology #mTORC1 #ProteinStructure #Biophysics #AAAplusATPase #SynapticPlasticity #Mitochondria
@naturepoker @thatdnaguy ha ha don't worry, data storage is also a hot mess in "professional" labs! 😅
The average academic group doing #cryoEM experiences exactly what you describe. The bottleneck is either storage, or how fast we can complete projects to the point when we can deposit datasets to a public database and free up storage space on our end.
The storage space used now to hold data for projects in the sloooow late phases of writing up and submitting, is literally preventing me from starting new projects... (which, in a way, is good when trying to finish projects... negative feedback loop and all that).
PhD student in cryo-ET of membrane trafficking at MPI-NAT in Göttingen, DE
Max-Planck-Institute for Multidisciplinary Science
Join our #CryoEM lab at MPI-NAT in Göttingen for a PhD in membrane trafficking
See the full job description on jobRxiv: https://jobrxiv.org/job/max-planck-institute-for-multidisciplinary-science-2777...
https://jobrxiv.org/job/max-planck-institute-for-multidisciplinary-science-27778-phd-student-in-cryo-et-of-membrane-trafficking-at-mpi-nat-in-gottingen-de/?fsp_sid=1131
PhD student in cryo-ET of membrane trafficking at MPI-NAT in Göttingen, DE
Max-Planck-Institute for Multidisciplinary Science
Join our #CryoEM lab at MPI-NAT in Göttingen for a PhD in membrane trafficking
See the full job description on jobRxiv: https://jobrxiv.org/job/max-planck-institute-for-multidisciplinary-science-27778...
https://jobrxiv.org/job/max-planck-institute-for-multidisciplinary-science-27778-phd-student-in-cryo-et-of-membrane-trafficking-at-mpi-nat-in-gottingen-de/?fsp_sid=914
Meharry Medical College Ph.D. candidate KeAndreya Morrison's highlight features a @Nature Communications publication from the laboratory of Christopher D. Lima whose work helped to uncover how the cell’s nuclear gateway controls molecular traffic and regulate protein function, offering new insights into gene regulation and disease.
Read more here: https://medium.com/sbgrid-community-news/dual-role-of-the-nuclear-gatekeeper-47013dcb1b05
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