Lmst

How can the #ubiquitin #ligase APC/C recognize and inactivate the major kinase, CDK1-Cyclin B1, as soon as the spindle checkpoint is switched off?
Luca Cirillo, Rose Young et al identify chromosome as the key platform, recruiting both Cyclin B1 and APC3 via arginine anchor motifs in their sequences
Jon Pines, Claudio Alfieri and coworkers
https://www.embopress.org/doi/full/10.1038/s44318-024-00194-2

#CryoEM shows the human #HECT domain-containing E3 #ligase #UBR5 forms an 𝛂-solenoid scaffold from an antiparallel dimer, and efficiently extends #ubiquitin chains on primed substrates such as #AKIRIN2
@haselbachlab @IMPvienna Nick Brown #UNC_PHCO
#RefereedPreprint ℅ @ReviewCommons
https://www.embopress.org/doi/10.15252/embj.2022113348

Extensive multivalent interactions between intrinsically disordered regions in the #BRCA1/#BARD1 #E3 #ligase and #nucleosome substrates facilitate #chromatin recruitment and contribute to histone H2A #ubiquitylation and #DNArepair
Sam Witus, Rachel Klevit, Weixing Zhao et al
https://www.embopress.org/doi/10.15252/embj.2023113565

Schematic visualization of the article's synopsis

How are specific dysfunctional #mitochondria removed under steady-state conditions?

Julia Pagan and coworkers (University of Queensland) show that an #SCF #ubiquitin #ligase based on #FBXL4, mutated in #MTDPS13, limits basal #mitophagy via #ubiquitination of #NIX and #BNIP3 receptors

https://www.embopress.org/doi/10.15252/embj.2022112767

What controls basal, #Parkin-independent #mitophagy?

E3 #ubiquitin #ligase screen by Sylvie Urbé, Michael Clague and coworkers identifies #VHL and #FBXL4 as negative regulators of mitophagy receptors #NIX and #BNIP3 via transcriptional and direct post-translational mechanisms, respectively

https://www.embopress.org/doi/10.15252/embj.2022112799

Hidden #RNA #repair mechanism discovered in humans.

#ligase #C12orf29 #CRISPR #chemoproteomic

https://phys.org/news/2023-04-hidden-rna-mechanism-humans.html

EMBO Journal Issue 4/23:
- Commentary on #creativity needs in research
- #Degron recognition in orphan complex subunits by CLR4-DCAF12 E3 #ubiquitin #ligase
- #Plasmamembrane topography of #IgM #Bcell receptors
- #YAP roles and regulation in #tumorigenesis
- #Brassinosteroid interplay with #katanin #microtubule severing

Cover by Satarupa Bhaduri, Analine Aguayo, Sonya Neal et al
https://www.embopress.org/toc/14602075/2023/42/4

EMBO Journal 4/23 cover features a cartoon depiction of yeast Derlin Dfm1 as a parcel service van, delivering an "Orm2"-labelled package to a household postal box that form part of a Golgi vesicle stack.

Viral mimicry of a #DCAF #ubiquitin #ligase adaptor: #CryoEM shows how rodent #cytomegalovirus #CMV E27/M27 hijacks host cell #CRL4 #cullin-RING E3s to degrade #STAT2 and suppress #antiviral #interferon signaling
https://www.embopress.org/doi/10.15252/embj.2022112351

#BRD4-#PRC2 represses #transcription of T-helper 2-specific negative regulators during #Tcell #differentiation:
Brd4 interacts with #Polycomb repressive complex 2 (PRC2) to repress transcriptional expression of T-helper 2 (Th2)-negative regulators #Foxp3 and E3 #ubiqutin #ligase #Fbxw7 during lineage-specific differentiation of #Th2 cells from mouse primary naïve CD4+ T cells
https://www.embopress.org/doi/10.15252/embj.2022111473

Protein complex assembly #qualitycontrol in action:
#cryoEM structures by Nico Thomä at FMI and colleagues show how #CRL4-#DCAF12 #ubiquitin E3 #ligase engages a C-terminal di-Glu #degron in CCT5 recognizable only when this subunit is not incorporated into the #TRiC/#CCT #chaperone complex
https://www.embopress.org/doi/10.15252/embj.2022112253

Unusual #ubiquitin #ligase interplay in #Hippo #signaling:
Bin Zhao, Xin-Hua Feng and colleagues Zhejiang University show that #HERC3 blocks #YAP/#TAZ recognition and degradation via #SCF-#betaTrCP independent of its own E3 activity
https://www.embopress.org/doi/10.15252/embj.2022111549

Discovery of a novel human #protein that functions as #RNA #ligase first report of such #protein in higher #eukaryotes | RNA ligases play an important role in #RNArepair, thereby maintaining RNA integrity. Any malfunction in RNA repair in humans seems to be associated with #diseases like #neurodegeneration and #cancer Discovery of a novel human protein (C12orf29 on chromosome 12) as an RNA ligase....