#Optogenetic manipulation of focal adhesion targeting shows that local #actomyosin traction force development induces focal #adhesion sliding and disassembly
Julien Aureille, Alexander Bershadsky et al
https://www.embopress.org/doi/full/10.1038/s44318-024-00114-4
#Lysosomes are crucial cellular degradation centers, but how can one selectively modulate their activity? This study describes novel #optogenetic tools for the light-inducible manipulation of lysosomal physiology in cells & in #Celegans #PLOSBiology https://plos.io/49WGds5
Research team develops new technique to release and study individual #proteins in #cells.
https://phys.org/news/2024-03-team-technique-individual-proteins-cells.html
#LITOS: A versatile #OpenSource #LED #illumination tool for #optogenetic stimulation:
-32 × 64 LED matrix
-#ESP32-controlled via #WLAN
-usable with different formats of multi-well plates, petri dishes & flasks.
https://doi.org/10.1038/s41598-022-17312-x
#DIYbio #lab #instruments #optogenetics
#Optogenetic stimulation of GABAergic projections from the Ventral Pallidum (VP) to the Ventral Tegmental Area (VTA) enhances reward reinforcement but not seeking in rats (#Neuroscience , #AffectiveNeuroscience )...
https://www.cell.com/cell-reports/pdf/S2211-1247(23)01680-7.pdf
Opto-RhoGEFs is an #optogenetic toolbox that can help researchers visualise and manipulate the barrier between endothelial cells like the ones in this clip. #12DaysOfeLife #Optogenetics https://elifesciences.org/articles/84364?utm_source=mastodon&utm_medium=social&utm_campaign=organic_12Days
Using a sophisticated #optogenetic approach, the researchers have discovered a previously unknown #endogenous acid sensor in #plant #cells. And they have discovered in the guard cells of leaves that there is a #calcium store that plays an important role in processing #proton signals in #cellular responses.
#Biology #sflorg
https://www.sflorg.com/2023/12/bio12152301.html
New work by Sanzeni et al. (2023): "Mechanisms underlying reshuffling of #visual responses by #optogenetic stimulation in mice and monkeys"
🌍 https://www.cell.com/neuron/fulltext/S0896-6273(23)00706-7
#OptoPi: An #OpenSource #3Dprinted flexible platform for the analysis of small #animal #behaviour:
-customisable #VIS/#IR #illumination, allows for #optogenetic stimulation
-#imaging by #RaspberryPi #camera
-#Arduino control
https://doi.org/10.1016/j.ohx.2023.e00443
#DIYbio #lab #instruments
August's most-read Tools paper presents an #optogenetic toolbox to reversibly control Rho #GTPase activity for manipulating the barrier between #endothelial cells: https://elifesciences.org/articles/84364?utm_source=mastodon&utm_medium=social&utm_campaign=submissions_organic
To migrate efficiently, neutrophils must polarize their cytoskeletal regulators along a single axis of motion. @JasonPTown & @OWeinerLab use spatially-defined #optogenetic control of a leading edge organizer to explore how #neutrophil-like cells balance “decisiveness” (needed to polarize in one direction) with flexibility (to respond to new cues) #PLOSBiology https://plos.io/3ZAldnx
Conversations with #plants: Can we provide plants with advance warning of impending dangers?
#biosensors #signalling #ABACUS2 #GPS1 #CcaS_CcaR #optogenetic
https://phys.org/news/2023-09-conversations-advance-impending-dangers.html
How does the brain consider an animal's current actions when responding to its environment? @AndrewLeifer &co use high-throughput #optogenetic perturbations to show how motor-related signals influence a #nematode’s response to #TouchSensation. #PLOSBiology https://plos.io/455hWOh
.@xanderjones82 &co describe Highlighter, an #optogenetic tool for fine-scale eukaryotic #GeneExpression control, and deploy it in #plants to enable cell-level hypothesis testing. @slcuplants @RobertoHofmann1 #PLOSBiology https://plos.io/3RvZvzh
#optoPlate-96: An #OpenSource #GUI to design high-throughput #optogenetic experiments with the optoPlate-96:
Paper: https://doi.org/10.1038/s41596-020-0349-x
GitHub: https://github.com/WeberSynBioLab/optoConfig-96
#DIYbio #lab #instruments #Python #Arduino #fluorescence #PlateReader #optogenetics
#optoPlateReader (#oPR): An #OpenSource #Arduino-controlled #PlateReader that allows #automated #optogenetic stimulation & #spectroscopy in each well of a 96-well plate:
https://doi.org/10.1101/2022.07.13.499906
#DIYbio #biohacking #chemistry #lab #instruments #fluorescence #OD #LED #python #GUI
Our favourite #Platynereis #opsin now in the mammalian brain as a new #optogenetic tool!
"Here we ... found that the Platynereis dumerilii ciliary opsin ... is an efficient, versatile, light-activated bistable GPCR that can suppress synaptic transmission in mammalian neurons with high temporal precision in-vivo "
🚨#Optogenetic preprint alert🚨
Check out PdCO:
Light-controlled inhibition of synaptic transmission via the inhibitory #GPCR pathway. #optogenetics #neuroscience
Link:
A clinically viable approach to restoring visual function using #optogenetic #gene #therapy https://www.cell.com/molecular-therapy-family/methods/fulltext/S2329-0501(23)00067-0 "Chronos-expressing vectors were efficacious over a broad range of vector doses and stimulating light intensities, and were well tolerated"; #blindness
New #Neuroscience paper, with potential implications for the pyramidal-cell specificity of some #Optogenetic stimulations:
“our results challenge the use of CaMKIIα promoter-driven protein expression as a selective tool in targeting cortical glutamatergic neurons using viral vectors”
CaMKIIα Promoter-Controlled Circuit Manipulations Target Both Pyramidal Cells and Inhibitory Interneurons in Cortical Networks
#NeuroPaper #Optogenetics
