And we need to end with some cat content for my amazing, brilliant, and fascinating co-first authors Oda Schiøtz and @CJOKaiser and who are both proud cat parents (displayed is actually Christoph's cat 😁)

Woooohoooo! :)
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RT @CJOKaiser
It‘s out on biorxiv!

Thanks to Oda H Schiøtz, @svenklumpe, @DustinMorado Matthias Pöge, @jon___schneider Florian Beck and Christopher Thompson for the productive work! Amazing colleagues, and Juergen M. Plitzko steadily supporting and enabling it all!

https://www.biorxiv.org/content/10.1101/2023.04.28.538734v1
https://twitter.com/CJOKaiser/status/1652825947681202176

Thanks to all the co-authors @DustinMorado @jon___schneider @MatthiasPoge & twitterless Florian Beck + Christopher Thompson @thermosciEMSpec .

Thanks to all the co-authors @DustinMorado @jon___schneider @MatthiasPoge & twitterless Florian Beck + Christopher Thompson @thermosciEMSpec .

It's been really stressful the last couple days, we locked ourselves in the seminar room finishing this story. After some nice celebration it is finally sleeptime😴

We anticipate the method to enable the study of multicellular organisms and tissues by cryo-electron tomography. To do the proof-of-princple, take a look at this nice ribosome at 6.9 Å!

If we got you interested and you want to know more on how we sample molecular anatomy in whole organisms, check out the pre-print here: https://www.biorxiv.org/content/10.1101/2023.04.28.538734v1

This enabled us to extract HUGE chunks and obtain tons of lamellae from them. It revealed all the beautiful anatomy that the C. elegans L1 larva has to offer. We are only highlighting a few here: nerve ring, pharny, body wall muscle, hypodermis and many more!

By using a copper adapter attached to the EasyLift needle, we have enabled lift-out of large volumes. We go on to deploy this on C. elegans L1 larvae frozen by a slightly adjusted method based on the amazing Waffle method @SEMC_NYSBC

Glad to finally share our manuscript on “Serial Lift-Out” by the amazing twitterless Oda Schiøtz, Jürgen Plitzko, @CJOKaiser , and myself. Doing multiple sections from single lift outs showing beautiful C. elegans ultrastructure 🤯 I could watch it all day 🤩How did we get there?

Drosophilst probably know this already but I just digged out and fell in love with this beauty 🤩, the first recorded description of #Drosophila: Johann Wilhelm Meigen (1830) “Systematische Beschreibung der bekannten europäischen zweiflügeligen Insekten”(https://www.biodiversitylibrary.org/item/132636#page/430/mode/1up)

Glad that the participants of #EMBOinsitu voted for me to get this prize! What directly pops into the head though: Does democratic voting of poster prizes feed into gender bias? Maybe worthwhile to collect data on @EMBLEvents and maybe adjust policy accordingly ? :)
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RT @EMBLEvents
Check out the best posters from 'In situ structural biology: from cryo-EM to multi-scale modelling'. 👀Congratulations to our five poster prize winners Joe…
https://twitter.com/EMBLEvents/status/1634185996752281603

RT @Beck_Laboratory
(1/3) New preprint by @HuaipengXing @Reiya_Tan1guch1 @iskhusainov @jpkreysing @sonjawelsch and twitterless Beata Turonova visualizes the translation elongation cycle in intact human cells. #CryoEM #teamtomo @MPIbp
https://biorxiv.org/cgi/content/short/2023.03.02.529652v1

Disclaimer: So far, only the FIB operations and correlative milling with 3DCT have been developed. There is great software out there to collect volume imaging data on CrossBeams, thus it is not high on the priority list. You can change that by contacting me with a good reason 😅

Check it out @PilhoferLab @GregorWeiss1 @williamnwan and everyone else using @zeiss_micro cryo-FIB/SEM solutions out there! Let me know how it goes 😁 Big thanks to @Beck_Laboratory and Twitterless Jürgen Plitzko for the support. Happy milling!

Great experience to code with Roland Salzer (Zeiss Solutions Lab) and shoutout to Andreas Schertel & Endre Majorovits @zeiss_micro and Zhengyi Yang & Julia Mahamid @embl for help on the CrossBeam operation and providing microscope time in Oberkochen and Heidelberg, respectively.

We listened to the community and here is the result: Got a CrossBeam that you would like to use for automated milling? The driver for lamella workflows in SerialFIB (https://github.com/sklumpe/SerialFIB/tree/dev), developed in collaboration with @zeiss_micro , has now been tested on 2 Crossbeams

Except for the CrossBeam operation (which I assume proud owners will be very familiar with), everything on how to set up positions is covered in the SerialFIB Tutorial on YouTube: https://www.youtube.com/watch?v=QR7ngJ0apBk as well as summarised here: https://github.com/sklumpe/SerialFIB/blob/main/20210428_SerialFIB_Tutorial.pdf

One in Oberkochen and one in Heidelberg. Installation to be simplified, but if you would like to try it on your system, the code + description are online (https://github.com/sklumpe/SerialFIB/tree/dev). As I only rarely have access to the microscopes, please let me know if you run into problems!

It’s been very inspiring to visit GMI @viennabiocenter and present my research. Thank you for the invite @ramundo_silvia!!! Simply amazing to discuss science with @plaschka_lab @haselbachlab @PamelaVetrano @juliusbrennecke @PauliGroup @BalzarottiFran @PlantoPhagy @ShotaroOtsuka