PSA: The restriction enzyme DpnI is a great way to remove the plasmid template after a PCR reaction.
It has a 4-base recognition sequence and only cuts methylated or hemimethylated DNA. Therefore, it will chop up almost any plasmid template but not digest the PCR product. It also works in most polymerase buffers.
Sustainability tip for yeast labs:
when replica plating you can combine two different dissection plates into one replica plate. This will reduce the number of plates you use by almost ½.
Here’s how it works.
As you know, a normal dissection takes up an entire plate, and then to genotype you need to replica plate to many (5) different plates—each reporting on a different marker.
To me, that’s a lot of plates.
🧵 /1
Most frighting lab ‘hack’ I ever saw was someone super gluing a cracked chloroform bottle. The best is using a cut-up rubber glove as a hair tie #LabHacks #LifeHacks
Thinking of getting either a Google or Alexa smart speaker for the lab (mostly so labbies can listen to music at the bench) - any thoughts/experience on which is better? Any good lab hacks I havn’t thought of yet that could make this even more useful? I have avoided them like the plague at home so I have no opinions…
No idea what hashtags to put on this… #LabHacks #LabChat #RealTimeScience #WetLab #CellBiology
