#luciferase

Brian Englishsciencethecat
2025-04-05

Dr. Eric Schreiter demo at @hhmijanelia

2024-06-20

#Luciferase-Assays sind Standard in vielen Zell-Labors, die kommerziellen Kits sind jedoch keine Schnäppchen. Andrea Pitzschke beschreibt, wie man mit einem hauseigenem Lysepuffer und simplen Modifikationen die Assay-Kosten drosseln kann: laborjournal.de/editorials/302 #Labor #LaborTool

Comicbild eines lächelnden Glühwürmchens mit Laterne. Rechts daneben Überschrift "Preisoptimierter Luciferase-Assay" sowie Vorspann des im Post verlinkten Artikels.
2023-04-15

@PhiloNeuroScie

“To expand the repertoire of endogenous cellular communication, we developed a synthetic, photon-assisted synaptic transmission (PhAST) system. PhAST is based on luciferases and channelrhodopsins that enable the transmission of a neuronal state across space, using photons as neurotransmitters.”

Porta-de-la-Riva et al. 2023 nature.com/articles/s41592-023

What a fun project and cool finding, and made in #Catalunya to top it off at the Photonic Science Institute.

Combine that with #photopharmacology and neural circuits may be able to dose themselves according to activity patterns.

#neuroscience #PhAST #Luciferase #ChannelRhodopsin #biophotonics #optogenetics

Nicola Romanònicolaromano@qoto.org
2023-03-20

I have created a little R #Shiny interface to deconvolute #luciferase assay data.

This is based on Brown 2008 - Inferring gene expression dynamics from reporter protein levels (but does not include volume correction)

The app also reports initial rate, maximum and decay rate

It is available here, for anyone to enjoy, modify etc

github.com/nicolaromano/LucDec

#rstats #analysis #biology #transcription

Example of a luciferase assay trace and the corresponding deconvoluted trace, which can be calculated through the Shiny app. A linear fit to the beginning of the deconvoluted trace is shown, to calculate initial rate, as well as a decaying exponential from the maximum point, used to calculate decay rate.
2023-02-16

Tuning plant #promoters using a simple split #luciferase method for quantifying transcription factor binding affinity | bioRxiv

biorxiv.org/content/10.1101/20

Marcus Marcius Marcellusmarcas@mastodon.ie
2022-12-09

Things to do #InsteadOfWorldCup, 20/29:

#Molecules — who doesn’t love ‘em? With the linked tool #MolView, you can visualize them in all sorts of ways and even create your own.

Here’s a structural image of #luciferase, which (if you can somehow manage to synthesize it in your buttocks) will make your arse glow in the dark.

Boost and add your own!

#NoWorldCup2022 #Boycott #FIFA #Qatar #CorruptWretches #ContraIlCalcioModerno #SCIENCE! #GlowInTheDarkArsesFTW

molview.org

A structural model of luciferase

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