#PUFFFIN

2025-12-05

A special day as we celebrate the successful viva of Dr Tamina Lebek, who developed her brilliant #PUFFFIN cell-neighbour-labelling system during her PhD.

I must say we've outdone ourselves this time with the cake (credit: Rosa), and the amazing hat (credit: Jen, Sophie, Aisling, Alicia) topped with a felted pufffin that I'm very proud to say I made myself (also shown here in its embryonic form).

#LifeOfPI #PUFFFIN #Puffins

A slightly wonky puffin made by felting wool, sitting on the top of a cardboard hatA cake, representing Bass Rock near Edinburgh, covered in Puffins made of sugar with a sign saying "Congratulations Dr Lebek".  The cake is covered in white icing and coconut, representing all the seabird-pooh that covers Bass Rock.Dr Tamina Lebek holding a glass of bubbly and wearing her amazing hat made from card, covered in pictures of her PhD days, topped with a felted puffin and a cardboard puffin-decoy (long story) plus lots of fluorescence sparkles made of pipecleanersThe embryonic form of my felted pufffin. It is a basket with bits of coloured unspun wool in. I realise I should be celebrating Tamina but I'm actually mostly celebrating the fact I managed to make a pufffin.
2024-10-17

Excellent short video by Tamina Lebek explaining her new neighbour-labelling technology and her dreams of a PUFFFIN zoo!

youtu.be/jEG60WvEh6M?feature=s

#DevBiol #PUFFFIN #ProudSupervisor

2024-09-16

As summer ends, puffins leave the islands around Scotland and migrate long distances, heading westwards across the Atlantic, or southwards to Africa, and with some individuals venturing into the Mediterranean Sea.

This one ended up on the cover of today's @embojournal 😃

embopress.org/toc/14602075/43/

#DevBio #PUFFFIN #GotTheCover

The cover of the 16 Sept edition of EMBO Journal, featuring an image of fluorescent cells that have been arranged to form the shape of a puffin. The strapline reads "Illuminating cellular neighbourhoods with PUFFFIN"
2024-07-16

..Neighbour-labelling in intact tissues!

Here we benefited from outstanding collaborators

Giulia Boezio and James Briscoe at the Crick used #PUFFFIN to achieve neighbour-labelling in chick embryos (new Fig5) #PUFFFHEN

and Lida Zoupi used lenti-PUFFFIN to demonstrate neighbour labelling in mouse brain slice cultures (new Fig EV5) #PUFFFMOUSE

and…

11/n

Part of a figure from a paper showing a cartoon of a chick embryo indicating site of electroporatoin in the caudal ectoderm. A second panel shows fluorescent images focusing on the electroporated region after 24h. These panels show a single red cell (PUFFFIN secretor) surrounded by a local neighbourhood of cells that are decorated with orange sparkles (PUFFFIN labelled neighbours). More distant cells are visualised in blue with DAPI but do not have any orange sparkles.
2024-06-18

I'm completely in love with the images that Tamina Lebek and I have just submitted to be #NotTheCover? #MaybeTheCover! #FingersCrossedForTheCover🤞

#PUFFFIN
#LifeOfPI

2024-06-11
2023-10-10

"And now my tummy's beginning to rumble...."

We had great success combining our #PUFFFIN system with HALOtag technology to achieve super-bright colour-of-choice neighbour-labelling

We had rather less success resisting the temptation to name this "PUFFHalo"

So, Rosa made the most amazing Puffhalo Crumble cakes for yesterday's lab meeting. The eyes of a gruffalo, the beak of a puffin, topped with a delicious crumble #CakesOfMastodon

A cake with the eyes of a gruffalo and the beak of a puffin and a green pimple, depicted in sweets

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