Lmst

#TMEJ

The PLK1 kinase mandates DNA polymerase theta to safeguard the #genome during #mitosis.

#preLighter Pierre Caron covers a #preprint by Camille Gelot #institut_curie and team that redefines the physiological function of #TMEJ.

#DNArepair #GenomeStability
https://prelights.biologists.com/highlights/pol%ce%b8-is-phosphorylated-by-polo-like-kinase-1-plk1-to-enable-repair-of-dna-double-strand-breaks-in-mitosis/

(A) Representative micrographs of immunofluorescence showing GFP-tagged Polϴ foci (green) localizing at mitotic DNA breaks (gammaH2A.X, Red) in human cells following replicative stress induced by the inhibition of ATR (ATRi). (B) Representative micrographs of either WT GFP-Polϴ or the GFP-PhD-Polϴ mutant in interphasic and mitotic cells. While the wild type of Poltheta (WT- Polϴ) is recruited to DNA breaks in interphase and mitosis, the mutant form that cannot be phosphorylated by PLK1 (PhD-Polϴ) is not recruited to mitotic DNA breaks.

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