#preLighter

2025-03-27

We bumped into #preLighter and former intern (also @the_node & @focalplane_jcs) Ryan Harrison. ๐Ÿ™Œ #biologists100

Here, he presented a beautiful (pink ๐Ÿคฉ) poster detailing his work on cell organisation & 3D shape emergence in a posterior neuruloid system. โœจ

#organoids #development #ECRs #preprints

2024-08-08

On how membrane curvature allows our hearts to beatโ€ฆ ๐Ÿ’“

#preLighter Barbora Knotkova highlights beautiful work from Yang Yang & colleagues #preprint

#preLight + author response โฌ‡๏ธ ๐Ÿ‘€

prelights.biologists.com/highl

#biology #heart #cells #curvature #membranes

2023-06-05

The PLK1 kinase mandates DNA polymerase theta to safeguard the #genome during #mitosis.

#preLighter Pierre Caron covers a #preprint by Camille Gelot #institut_curie and team that redefines the physiological function of #TMEJ.

#DNArepair #GenomeStability
prelights.biologists.com/highl

(A) Representative micrographs of immunofluorescence showing GFP-tagged Polฯด foci (green) localizing at mitotic DNA breaks (gammaH2A.X, Red) in human cells following replicative stress induced by the inhibition of ATR (ATRi). (B) Representative micrographs of either WT GFP-Polฯด or the GFP-PhD-Polฯด mutant in interphasic and mitotic cells. While the wild type of Poltheta (WT- Polฯด) is recruited to DNA breaks in interphase and mitosis, the mutant form that cannot be phosphorylated by PLK1 (PhD-Polฯด) is not recruited to mitotic DNA breaks.
2023-04-27

Missed the 9th International Symposium on the Biology of Vertebrate Sex Determination last week? #VSDHawaii2023

#preLighter Martin Estermann has put together a #preList containing #preprints that were discussed and presented there.

๐Ÿ“œ preList: prelights.biologists.com/preli

VSD2023 Conference Poster
2023-04-18

Helping funders & institutions as they consider #OpenScience implementation.

Kristen Ratan and colleagues discuss their approach at #ASAP_Research to incorporate open science policies and advance science through reuse and collaboration. #preprint

Great new #preLight from Kanika Khanna!

๐Ÿ‘‰ prelights.biologists.com/highl

#preLighter #ASAPbio_Fellow

Schematic of the compliance workflow for ASAP grantees.
2023-03-07

Roses are red, violets are blue, when the disk proper grows, hippo stretches peripodial epithelium too. ๐ŸŒน

A new post from Girish Kale covering a #preprint led by another #preLighter: Sophia Friesen who works in the #HariharanLab

Check it out! ๐Ÿ‘‰ prelights.biologists.com/highl

Diagram of the two layers of the wing disc in the third larval instar. The disc proper (DP, dark blue) is mostly composed of columnar cells and has characteristic buckles and folds (white lines); the peripodial epithelium (PE, light blue) is composed of squamous cells in the central region and cuboidal cells in more peripheral regions. Representative cell outlines of squamous cells are indicated. The diagram below shows a cross section through the wing disc along the dotted line above. Note the differences in cell shape between the two layers.
2023-01-31

Two new posts on preLights today! ๐Ÿ‘€

The first has been written by our new #preLighter Yueh Cho (postdoc at #IPMBSinica & #AcadSinica) who covers a #preprint from Tina Schreier and colleagues which shows that light and photosynthesis trigger plasmodesmal formation in C4 dicotyledons ๐ŸŒฑ

๐Ÿ“œ prelights.biologists.com/highl

#plants #grasses #dicotyledons #plasmodesmata

Heatmap of plasmodesmata distribution. Cell interfaces in high-resolution 2D SEM maps of C4 G. gynandra, C3 T. hassleriana and C3 A. thaliana leaf cross sections were coloured according to plasmodesmal frequency (number of plasmodesmata observed on the interface, divided by the interface length [ฮผm]).
2023-01-17

An inducible tagging strategy to label proteins enables nanometer-scale localisation of molecules in human cells. #cryo-ET #FluorescenceMicroscopy. ๐Ÿ”ฌ

๐Ÿ“œ prelights.biologists.com/highl

#preLighter Martyna Kosno-Vega covers an exciting #preprint from the #MahamidLab #EMBLHeidelberg.

#biochemistry #bioengineering #imaging #biophysics

2023-01-04

Happy New Year! ๐ŸŽ† Two preLights to kick off 2023.

2nd: urban-adapted mosquitoes may spread malaria to new regions as the Earth warms ๐ŸฆŸ๐ŸŒŽ - an important (and alarming) #preprint from Sadie J. Ryan, Catherine A. Lippi and colleagues.

By #preLighter Sophia Friesen #HariharanLab #Berkeley

๐Ÿ“œ prelights.biologists.com/highl

Thermal suitability for transmission of P. falciparum and P. vivax malaria by An. stephensi.Transmission suitability is shown under current climate conditions, and for the year 2050 at RCP 4.5 and RCP 8.5. The number of months of suitable temperatures are given as shaded areas, where the posterior probability of S(T)>0 is 0.975.
2023-01-03

Happy New Year! ๐ŸŽ† Two new #preLights to kick off #2023.

1st: Itโ€™s all clicking together - Keqing Nian, Yifang Liu, Laura Brigandi & Sara H. Rouhanifard develop a DNA oligomer-conjugated ligand for copper-catalysed azide-alkyne cycloadditions.

By #preLighter @zhanghe_goh #EPSRC #CDT #Cambridge

๐Ÿ“œ prelights.biologists.com/highl

Advantages of the authorsโ€™ BTT(1,2)-DNA over current methods.
2022-11-25

Check out the latest #post @preLights? ๐Ÿ“œ๐Ÿ‘€

prelights.biologists.com/highl

#preLighter Nadja Hรผmpfer covers a #preprint which describes the combination of #SRRF analysis with 10-fold expansion microscopy #ExM. ๐Ÿ”ฌ Their approach allows a final resolution of 1 nm; a #resolution at which the shape of individual proteins becomes apparent when labelled with a pan-protein staining.

This preLight also includes comments from #authors Ali H. Shaib & Silvio O. Rizzoli ๐Ÿ“

#ONE_Microscopy #immunoglobulin

ONE analysis of single molecule - in this case an isolated immunoglobulin.
2022-11-23

Navigating a tense situation: the plasma #membrane and actin #cortex form an integrated system for rapid long-range #tension #propagation.

๐Ÿ“œ prelights.biologists.com/highl

#preLighter Nicolaes Hyun-Kee Min covers a beautiful #preprint from @HenryDeBelly and team (Weiner, Turlier, and Bustamante labs) which provides a simple and widely applicable #model of how tension propagates. One that not only considers membrane rigidity/fluidity, but the underlying actin cortex as well.

Experimental system to simultaneously measure membrane tension while optogenetically inducing actin protrusion.

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