#Paper_alert

Liao and Shattil
"Talin, a Rap1 effector for integrin activation at the plasma membrane, also promotes Rap1 activity by disrupting sequestration of Rap1 by SHANK3"

@J_Cell_Sci

https://journals.biologists.com/jcs/article/doi/10.1242/jcs.263595/365447/Talin-a-Rap1-effector-for-integrin-activation-at

#paper_alert
Min et al.,
From Static to Dynamic Structures: Improving Binding Affinity Prediction with Graph-Based Deep Learning

https://onlinelibrary.wiley.com/doi/10.1002/advs.202405404

Dynaformer predicts the binding affinities by learning the geometric characteristics of the protein-ligand interactions from the MD trajectories.

#paper_alert
Cellular stiffness sensing through talin 1 in tissue mechanical homeostasis
Chanduri et al
https://www.science.org/doi/10.1126/sciadv.adi6286

#paper_alert #review
The desmosome comes into focus
Navaneetha Krishnan Bharathan
Alexa L. Mattheyses
Andrew P. Kowalczyk
@JCellBiol
https://rupress.org/jcb/article/223/9/e202404120/276896

#paper_alert
αMI-domain of integrin Mac-1 binds the cytokine pleiotrophin using multiple mechanisms

Nguyen et al
https://www.cell.com/structure/abstract/S0969-2126(24)00139-4
@structure_cp
PTN’s N-terminal domain binds αMI-domains using a unique site on αMI-domain
PTN’s C-terminal domain binds active αMI-domain through the MIDAS metal

Cryo-EM architecture of a near-native stretch-sensitive membrane microdomain.
Kefauver et al.
Structure of near-native eisosomes helical tubules made up of BAR-domain proteins Pil1 and Lsp1
https://www.nature.com/articles/s41586-024-07720-6
#paper_alert

#paper_alert
Liu et al
Cortactin stabilizes actin branches by bridging activated Arp2/3 to its nucleated actin filament

Cortactin binds to the daughter actin filament. Cortactin preferentially binds to activated Arp3 and stabilizes F-actin-like interface of activated Arp3

Congrats to the Way and Moores labs

https://www.nature.com/articles/s41594-023-01205-2?utm_source=nsmb_etoc&utm_medium=email&utm_campaign=toc_41594_31_5&utm_content=20240521

#paper_alert
Engineering an artificial catch bond using mechanical anisotropy

Liu, Z., Liu, H., Vera, A.M. et al. Nat Commun 15, 3019 (2024).

https://www.nature.com/articles/s41467-024-46858-9

#paper_alert

High-throughput prediction of protein conformational distributions with subsampled AlphaFold2
Monteiro da Silva et el
https://www.nature.com/articles/s41467-024-46715-9

And the link to the Colab notebook
https://t.co/EJ92Mmocol

#paper_alert
The focal adhesion protein talin is a mechanically gated A-kinase anchoring protein

M. Kang, Y. Otani, Y. Guo, J. Yan, B. Goult and AK. Howe

This work shows that PKA binds yo talin1 rod domain in a force-dependent manner, which couples cellular tension to cellular communication

https://www.pnas.org/doi/10.1073/pnas.2314947121

Explained in the following video by Ben Goult
https://m.youtube.com/watch?v=J42J5dzMCdY

#paper_alert
Generalized biomolecular modeling and design with RoseTTAFold All-Atom
https://www.science.org/doi/10.1126/science.adl2528

"RoseTTAFold All-Atom (RFAA) combines a residue-based representation of amino acids and DNA bases with an atomic representation of all other groups to model assemblies containing proteins, nucleic acids, small molecules, metals, and covalent modifications"

#paper_alert
Pip5k1γ regulates axon formation by limiting Rap1 activity
Di Meo et al @lsajournal
https://www.life-science-alliance.org/content/7/5/e202302383
Impairing Pip5k1γ's function or manipulating membrane tension by osmotic shock induces the formation of supernumerary axons by a hyper-activation of #C3G & #Rap1

#paper_alert
DynamicBind: predicting ligand-specific protein-ligand complex structure with a deep equivariant generative model
Lu W et al (2024) Nat Comm 15,1071
https://www.nature.com/articles/s41467-024-45461-2

#paper_alert

Esteban-Hofer et al.
Ensemble structure of the N-terminal domain (1–267) of FUS in a biomolecular condensate:

Biophysical Journal @biophysj
https://www.cell.com/biophysj/fulltext/S0006-3495%2824%2900039-0

Interesting application of electron paramagnetic resonance (EPR DEER) to characterize biomolecular condensate formed by liquid-liquid phase separation LLPS.

Work by the group of Gunnar Jeschke

#paper_alert
RAS G-domains allosterically contribute to the recognition of #lipid headgroups and acyl chains.
Work by Neha Arora, Yong Zhou and colleagues
https://t.co/DJKwGMMtum

#paywalled