Organic chemistry passionate, all kind of fluorescent stuff, dad, mountain addict.
@Spirochrome co-founder
🤯250 STED frames at 0.5 Hz with no bleaching and no phototoxicity!
Watch these HeLa cells stained with PKmito ORANGE, imaged by STED with a very short pixel dwell time and AI denoised.
Jaw breaking!
find out more in this cool manuscript : https://biorxiv.org/content/10.1101/2023.01.26.525571v1
Have a good #FluorescenceFriday with this live HeLa cell imaged by #STED. Actin in white and mitochondria in "Duo Intense Yellow" LUT by James Manton.
🙏 Image Credit: Tianyan Liu
📢New product launch!
We introduce SpyRho 555 from the lab of Murat Sunbul in Heidelberg. SpyRho 555 is a fluorogenic dye binding to RhoBAST aptamer for live or fixed cell RNA imaging. It works for widefield, confocal, SIM, STED and PAINT-like SMLM.
SpyRho 555 product page: https://spirochrome.com/product/spyrho/
SpyRho BioRxiv preprint link: https://www.biorxiv.org/content/10.1101/2022.10.24.513449v1
We thank Daniel Englert and
Murat Sunbul for providing the images and the helpful discussions.
📢PKmito™ probes are available!
And to launch these in style, a 50% DISCOUNT on all PKmito™ products is available from our distributors network until March 31st 2023.
Don't miss your chance to try these outstanding mitochondrial probes.
Learn more about PKmito™ probes on our website: https://spirochrome.com
#PKmito #mitochondria #LifeSciences #imaging
We are very grateful to
Tianyan liu and the
@zhixingchen lab for these amazing tools and images.🙏
📢The amazing PKmito RED, ORANGE and DEEP RED probes will be available next Monday (Jan. 16th) from all our distributors!
We will announce a cool promotion for the launch of the probes. Make sure to follow us to be in the loop next Monday!
#PKmito #mitochondria #Imaging #lifesciences #STED #microscopy
📢A new FastAct™ probe is available!
We proudly introduce SPY700-FastAct
https://spirochrome.com/product/spy700-fastact/
Our FastAct™ probes capture fast actin structures.
The movie shows 2 cell populations, stained separately with our SPY-DNA probes, that were subsequently mixed together and further stained with SPY700-FastAct, allowing to track the 2 populations based on the color of the nuclei.
We are extremely grateful to Maxime Cammeraat from the Margadant lab in, Amsterdam for providing the raw images.
Last #FluorescenceFriday of the 2022 !
Let's have a look a this beautiful collective #CellMigration with a staining of the organelles and actin cytoskeleton.
(Hey @Spirochrome you know how to make probes, huh neat staining with FastAct)
#scicomm @cellcommlab @focalplane_jcs
RIP la Râpe 😥
"Swiss made" isn't what it used to be...
Hope that I will be able to get a replacement part from the manufacturer.
Last #FluorescenceFriday for 2022!
The whole Spirochrome team whishes you a happy Holiday Season🎄and great New Year celebrations 🥳
We are very much looking forward to 2023 to tell you more about our exciting new products to come!
Image Credits:🙏Tianyan Liu PKmito ORANGE & SPY650-tubulin
Day 302 of my 3 day war.
100,000 of my soldiers are dead.
I remain a master strategist.
Old-school technique meets cutting-edge approaches! Finish this eggnog, fix your parents' TV and stay tuned 📺
@christlet shiiiiiiiiiiiiit... get well soon Christophe.
All of this started because I wanted an excuse to do what we lovingly refer to as the puffs assay - high-speed TIRF imaging of pH-sensitive GFP-tagged receptors that allows us to visualize receptor recycling as bright puffs of fluorescence.
For those of you wondering, #Mastodon is absolutely buzzing right now. Genuinely feels like we've hit critical mass and the feeds are getting informative & fun. Join us if you haven't. And use the Tusky or Toot app instead of the web interface.
My new standard to decide accepting or not review requests from journals.
We are thrilled to announce that the outstanding mitochondrial probes PKmito ORANGE , RED and DEEP RED will be available at Spirochrome through its distributors network in January 2023.
PKmito probes are gentle to the cells and allow long term imaging as well as recording amazingly crisp STED images of mitochondrial cristae.
Read more about PKmito ORANGE in the fresh article from Zhixing Chen and Stefan Jakobs labs out in @PNASNews
https://www.pnas.org/doi/10.1073/pnas.2215799119
#mitochondria #LiveCellimaging #PKmito
Big step - migrating my #cooking adventures here... Sticky coconut chicken and rice tonight https://cooking.nytimes.com/recipes/1023047-sticky-coconut-chicken-and-rice
Is #MicroscopyMonday a thing here? This image shows a single #neuron where #actin has been tagged (orange), with all neurons of the culture labeled for #microtubules in the cell body and dendrites (map2, blue), and for actin (gray)
