#VolumeEM

2025-05-16

From Elizabeth Marin at Zoology Dept., Cambridge University:

"Together with Greg Jefferis (MRC LMB, Cambridge), Wei-Chung Allen Lee (Harvard Medical School), and Meg Younger (Boston University), I have secured a £4.8M Wellcome Discovery Award to generate a mosquito brain connectome and investigate chemosensory circuits involved in human host-seeking."

"We are currently recruiting for two research assistant positions based in the Zoology department at Cambridge University. Please share this post with any likely candidates :)."

jobs.cam.ac.uk/job/51256/

#neuroscience #connectomics #mosquito #VolumeEM

2025-05-04

"Comparative connectomics of Drosophila descending and ascending neurons", Tomke Stürner et al. 2025 (Greg Jefferis and Katharina Eichler's labs).
nature.com/articles/s41586-025

Compares between males and females.

#neuroscience #Drosophila #connectomics #vEM #volumeEM

ZooCELL MSCA-Doctoral networkZooCELL@biologists.social
2025-03-20

We had our very first practical school at the Centre for Organismal Studies at @uniheidelberg !

This was a hands-on volume EM and sample preparation course with several theoretical lectures.

cos.uni-heidelberg.de/en/resea

Here is a short write up about the event by @abilgk

zoocell.eu/2025/03/13/practica

#microscopy #volumeEM #biology #science

A high pressure freezing instrument is being used to prepare samples for electron microscopy by participants of the ZooCell EM sample preparation course.
2025-03-06

🚀 Starting in a few hours! Join leading experts as they share insights on handling large #VolumeEM datasets & improving collaborative workflows.

📅 March 6 | 5-7PM CET | Online | Free
🔗 Register now: buff.ly/LaZw4aA.

ZooCELL MSCA-Doctoral networkZooCELL@biologists.social
2025-03-04

More #introduction about our ZooCELL network.

We will do a lot of volume electron #microscopy #volumeEM focusing on sensory systems in marine #animals

We will combine this with single-cell #genomics, correlative LM/EM, #AI -based image segmentation and classification, and genetics

You can read more about the participating labs here:

zoocell.eu/consortium/#Benefic

We will post about events, projects etc. in the coming years.
If you like #zoology #evodevo #marine #biology follow/boost us!

2025-03-03

⏳ 4 more days to go! Don't miss our online symposium on #VolumeEM data challenges - practical solutions for massive datasets & collaboration.

📅 March 6, 2025 | 5-7PM CET | Free registration
🔗 Sign up: buff.ly/LaZw4aA.

2025-01-16

Join our online symposium on #VolumeEM data challenges! Experts will share practical solutions for handling massive datasets & improving collaboration.

📅 March 6, 2025 | 5-7PM CET | Online | Free registration

Sign up for free at buff.ly/42gkyv4.

EuroBioImagingEuroBioImaging
2024-12-16

Join us for the last Pub before the Winter Break! ☃️
We'll learn about approaches from the experts at ’s Advanced Light & Electron Prague Node, part of the Volume EM community.
🗓️Fri, Dec 20 @ 13:00 CET
All are welcome 🔽
eurobioimaging.eu/events/diver

Norman Rzepkanormanrz
2024-10-21

On my way to . Looking forward to talking about large vEM data and other fun challenges! @webknossos

2024-08-12

GridTape TEM with beam deflection to reduce stage movements and increase imaging throughput:

"Fast imaging of millimeter-scale areas with beam deflection transmission electron microscopy", Zhao et al. 2024

nature.com/articles/s41467-024

#TEM #GridTapeTEM #vEM #VolumeEM #connectomics #neuroscience #microscopy #ElectronMicroscopy

2024-06-28

@nanographs

Thanks for sharing this – will have to think about it. In our case, we built in-house the gridtape reel holders which amount to a custom stage with a mini-camera in it for identifying slots and controlling imaging. So we don't use the factory-supplied stage.

biorxiv.org/content/10.1101/65

#vEM #volumeEM #TEM

Schematic of the GridTape reel-to-reel stage at the level of the TEM objective pole-piece. Portions of the TEM column above and below in beige with dashed outlines. Scale bar 10 cm.
2024-06-26

True as always that the way to make software run faster is to make it do less operations. After all, CPUs can only execute a fixed number of operations per unit of time.

Here, I tweaked code for serial section registration that drops execution time from 27 seconds to 100 milliseconds: a 270x speed up.

All it had to do is to search for matching SIFT features in one image only within a predetermined radius centered on one SIFT feature in another image. Extremely effective for when e.g., the maximum translation is known.

The matching code using a KDTree:
github.com/acardona/scripts/bl

The test script:
github.com/acardona/scripts/bl

#FijiSc #java #jython #volumeEM #vEM

2024-04-03

From Moritz Helmstaedter on #RoboEM:

"In today’s AI research, any project lasting longer than 6 months is considered slow, if not outdated and overrun by history. RoboEM, with a concept and an approach that seem quite plausible, still took 5 years from idea to the fully evaluated tool reported here. This was only possible with the patient support of the Max Planck Society, which encourages long-term projects, and the tenacity of the first author. Details made all the difference. And, as often, dead ends had to be avoided efficiently. We had to abandon, for example, the idea of using steering variability to indicate branch points — a nice analogy to road intersections in car steering, but too far-fetched to work well enough in brain tissue data. Sometimes giving up beloved ideas is as important as following through on others. M.H."

nature.com/articles/s41592-024

#connectomics #volumeEM #vEM #neuroscience #MaxPlanckSociety

2024-03-18

We have now published a new and massively extended/reworked preprint of the whole-body #Platynereis larval #connectome with over 50 figures

biorxiv.org/content/10.1101/20

All the analyses, plots and figures should be reproducible in #rstats with the code provided:

zenodo.org/doi/10.5281/zenodo.

@zenodo_org

by querying our public #CATMAID database:

catmaid.jekelylab.ex.ac.uk

#neuroscience @biology #volumeEM
@biorxivpreprint

Anatomy of head neuropils and global organisation of brain connectivity in the Platynereis larva
2024-03-11

“Perception: How larvae feel the world around them” by Jimena Berni elifesciences.org/articles/967

… an insight piece on Andreas Thum’s lab work on mapping the sensory organs of the #Drosophila larva with electron microscopy:

“Morphology and ultrastructure of external sense organs of Drosophila larvae”
Richter et al. 2024 elifesciences.org/articles/967

#neuroscience #vEM #VolumeEM

2024-02-29

EM stack of a golgi ribbon in a glial cell of #Platynereis, from Benvenuto et al.,:
"Evolution of the ribbon-like organization of the Golgi apparatus"
with small contribution from our lab.
sciencedirect.com/science/arti
#CellBiology #volumeEM #cell

2024-01-30

Our new EU PhD Network #ZooCell is hiring! We have 12 PhD positions in Germany, France, UK, Italy and Sweden.

The broad topic is evolution of sensory cell types in animal diversity: multidisciplinary training in 3D cellular reconstruction (volume EM), multimodal data analysis and science outreach.

euraxess.ec.europa.eu/jobs/189

Please share!

If you are interested in a PhD in #volumeEM, comparative #connectomics etc. check out the projects and apply!

#biology #PhD #neuroscience

Journal of Cell ScienceJ_Cell_Sci@biologists.social
2023-10-25

Our ‘Cell Scientist to Watch’ interview is with Lucy Collinson, Head of the EM Science Technology Platform @thecrick. We discussed Lucy’s career, the #volumeEM revolution and the upcoming @J_Cell_Sci special issue that she is guest editing with @guijacquemet journals.biologists.com/jcs/ar

Lucy Collinson showing a selection of cheese at Neal's Yard Dairy. See the interview for more details!
2023-10-06

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