This ATP-dependent DNA ligase crystallized in the DNA-encircling conformation, arrested as a step 2 intermediate in the catalytic cycle with the adenylating cofactor transferred to the 5′-phosphate of the DNA nick #RhizobiumPhage #DNALigase https://doi.org/10.1107/S2053230X2500411X

On Thursday June 26th, from 2 to 4 p.m., the next Café Solutions organized by the French Association of Crystallography (#AFC) Young #Crystallographers will be devoted to AutoProc (examples and limits).

For this event, we'll be delighted to welcome Dr William SHEPARD, Beamline Scientist at the Synchrotron Soleil.

English speakers are more than welcome 😉
Here's the zoom link to the Café Solutions:➡️ https://u-bordeaux-fr.zoom.us/j/83502635374
@strucbio @ActaCrystF
@afmblab.bsky.social
#crystallography

The structure of an A. bisporus mannose-binding protein variant reveals that the C-terminal region has an impact on sugar binding and is important for the mannose-binding affinity #AgaricusBisporus #CarbohydrateBindingProtein #LectinLikeProtein https://doi.org/10.1107/S2053230X25003905

How iron helps collagen enzymes work - and what happens when it doesn’t #PostTranslationalModifications #DNAViruses #Dioxygenases https://doi.org/10.1107/S2053230X25003735

This focused issue of Acta Crystallographica Section F entitled Empowering education through structural genomics contains 16 articles written by early career researchers, many of whom are undergraduates or high-school students https://doi.org/10.1107/S2053230X25003930

Check out our latest Interview with Authors
https://youtu.be/xQGq7PfAHxs?si=Kg7UxSsdd-ml7DCq

Our June 2025 issue is now available! https://tinyurl.com/a3bze27b

This month's cover star is the crystal structure of the ATP-dependent DNA ligase from the Rhizobium phage vB_RleM_P10VF bound to a nicked DNA duplex. Find out more at https://tinyurl.com/bdex43bu

These 16 articles, co-authored by early career researchers, use real structural data from the Seattle Structural Genomics Center for Infectious Disease (SSGCID) to analyse protein structures, develop scientific writing skills and contribute to peer-reviewed publications.

Explore how access to high-quality structural biology data is transforming STEM education in this new focused issue: https://journals.iucr.org/special_issues/2024/educationsg/

The Acta Cryst F Section Editors interview Raphael Meneghello, Andrey Nascimento and Daniela Trivella, the authors of 'High-throughput protein crystallography to empower natural product-based drug discovery'. Read the article at https://tinyurl.com/336984j9

https://www.youtube.com/watch?v=xQGq7PfAHxs

The 37th #ECALunchtimeWebinar will take place May 22nd 2025 @ 1pm CET.
In this #webinar, a new project, GEMS, proposed and managed by all three general interest groups (GIG-01, GIG-02 and GIG-03) of the European Crystallographic Association, will be presented. This project is intended to include the whole community of 🇪🇺 crystallographers, both early-career and experienced ones, therefore strengthening and vitalising the European crystallographic community.

Please register for the webinar here: https://www.eventbrite.com/e/37th-eca-lunch-webinar-tickets-1367524244829?aff=oddtdtcreator

@strucbio @afmblab.bsky.social @ActaCrystF #CCP4 #crystalography

Remodeling of the Saccharolobus solfataricus GINS (SsoGINS)-RecJ unit (green and salmon) from the AlphaFold-Multimer predicted model of SsoGINS-SsoRecJ that is needed to generate a SsoCMG model comparable to eukaryotic CMG. Find out more at https://tinyurl.com/mvx3urkx

The structure of a newly designed protein featuring a rare left-handed βαβ motif could not be solved by molecular replacement using the AlphaFold2 model @ActaCrystF @IUCr #DeNovoProteinDesign #LeftHandedBetaAlphaBetaMotif #AlphaFold https://doi.org/10.1107/S2053230X25003097

The structure of the tetrameric GINS complex from S. solfataricus reveals a core structure highly similar to the previously determined GINS core structures of other eukaryotes and archaea #DNAReplication #GINS #MinichromosomeMaintenance https://doi.org/10.1107/S2053230X25003085

The complex structure of piperine with the PPARγ ligand-binding domain provides a greater understanding of the way in which piperine modulates PPARγ function and its potential as a candidate for treatment of metabolic disorders #PPARgamma #Piperine https://doi.org/10.1107/S2053230X25002377

Structural analysis of M. tuberculosis Rv2173 reveals a canonical class I diterpene synthase fold and supports its annotation as a short-chain product-length synthase #MycobacteriumTuberculosis #IsoprenylDiphosphateSynthase #Rv2173 https://doi.org/10.1107/S2053230X25002298

The use of high-throughput protein crystallography to directly capture bioactive natural products from unpurified biota chemical samples using protein crystals is described #NaturalProducts #HighThroughoutCrystallography #CrystallographicCapture https://doi.org/10.1107/S2053230X25001542

Our May issue is out! https://tinyurl.com/yfhz6nf3
On the cover: the co-crystal structure of piperine and peroxisome proliferator-activated receptor gamma ligand-binding domain provides insight into their interaction. Read more at https://tinyurl.com/4r6yx83x

The structure of A. thaliana cyclophilin 37 may help to decipher how plants regulate photosynthesis and protect against high light damage, potentially guiding future research in improving plant stress tolerance #ArabidopsisThaliana #cyclophilin37 https://doi.org/10.1107/S2053230X25001979

This article is a contribution to a focused issue of Acta Cryst F on empowering education through structural genomics, highlighting the contributions of early-career authors. Pictured: early career authors Chi Nguyen, William Fan, Sean Fisher, Krystal Matthews and Jordan Norman.