PuckerLab

Molecular Plant Sciences at Uni Bonn mps.uni-bonn.de

PuckerLab boosted:
Boas Puckerboas_pucker
2025-05-24

Excited to share the publication of MGSE, our new approach to genome size estimation, in BMC Genomics:
Mapping-based genome size estimation
doi.org/10.1186/s12864-025-116

@PuckerLab

Concept of MGSE for the estimation of a genome size based on a read mapping. See https://doi.org/10.1186/s12864-025-11640-8 for details
PuckerLab boosted:
Boas Puckerboas_pucker
2025-05-11

Just wrapped up my talk at the University of Mainz on "Exploring the Specialized Metabolism of Plants with Long Read Sequencing" πŸŒΏπŸ”¬ Great to share how nanopore sequencing helps decode complex plant pathways with ecological and biomedical potential. Thanks to the organizers and all who joined the discussion! @PuckerLab

Screenshot of slide "Exploring the Specialized Metabolism of Plants with Long Read Sequencing"
PuckerLab boosted:
Boas Puckerboas_pucker
2025-05-02

πŸŽ“ Our course concludes with student project presentations covering topics like genomic feature extraction, sequence analysis, and large-scale data wrangling.
πŸ‘‰ See the course materials on GitHub: github.com/bpucker/PyBo

@PuckerLab

Illustration of four diverse students presenting their final Python course projects in a classroom. A projector screen behind them displays the course title 'PYTHON COURSE' with four project topics: 'Extraction of Genomic Features', 'Development of Molecular Markers from Sequence Variants', 'Analysis of Large Citation Data Sets', and 'Cleaning of FASTA Files'. Two audience members are visible from behind, listening attentively. The students are holding laptops or notes and appear confident and engaged.
PuckerLab boosted:
Boas Puckerboas_pucker
2025-05-02

Cornflower vibrant color comes from a unique complex of anthocyanins, flavones, and metal ions (Fe³⁺, Mg²⁺), stabilized by pH. This metal-anthocyanin interaction shifts the color to blue. : lnk.tu-bs.de/9Zz2uG
(πŸ“Έ doi.org/10.1101/2025.01.31.635)

Picture of a vibrant blue flower of Centaurea cyanus. Reference: https://doi.org/10.1101/2025.01.31.635922
PuckerLab boosted:
2025-05-02

We are using plant genomics & transcriptomics to understand the genetics and the evolution of the specialized metabolism of plants. Many long read sequencing projects (#ONT) are going on in the lab. We also like reusing public data sets to answer biological questions. #Python is the dominant script language in the group. We use these scripts to perform analyses beyond the potential of existing tools. Check out our website and get in touch if you would like to join: izmb.uni-bonn.de/en/molecular-

Beautiful Poppelsdorf Palace in the Botanical Gardens belonging to the University of Bonn
2025-05-01

We are using plant genomics & transcriptomics to understand the genetics and the evolution of the specialized metabolism of plants. Many long read sequencing projects (#ONT) are going on in the lab. We also like reusing public data sets to answer biological questions. #Python is the dominant script language in the group. We use these scripts to perform analyses beyond the potential of existing tools. Check out our website and get in touch if you would like to join: izmb.uni-bonn.de/en/molecular-

Beautiful Poppelsdorf Palace in the Botanical Gardens belonging to the University of Bonn
PuckerLab boosted:
Boas Puckerboas_pucker
2025-05-01

Excited to share our latest preprint about CoExpPhylo:
doi.org/10.1101/2025.04.03.647.

Not long ago, Nele excelled at a course for life scientists and acquired crucial skills for this work

@PuckerLab

CoExpPhylo workflow. Details are described here: https://doi.org/10.1101/2025.04.03.647051
PuckerLab boosted:
Boas Puckerboas_pucker
2025-05-01

Excited to share that I’ve started a new position as Professor for Molecular Plant Sciences at the University of Bonn! 🌱
@PuckerLab has officially moved, and the team is growing. Looking forward to exciting research and new collaborations ahead!
πŸ”— mps.uni-bonn.de

Botanical Gardens and Poppelsdorf Palace
PuckerLab boosted:
Boas Puckerboas_pucker
2025-03-01

Our updated Victoria genome preprint now includes an 'Additional file 0.' Bioinformaticians, we thought you might appreciate thisβ€”what do you think?

doi.org/10.1101/2024.06.15.599
@PuckerLab

Experimental design and workflow. Figure reference: https://doi.org/10.1101/2024.06.15.599162
PuckerLab boosted:
Boas Puckerboas_pucker
2025-03-01

While the Botanical Garden is quiet in winter, here is a stunning shot of the giant water lily, Victoria cruziana! 🌿 Curious about the molecular secrets behind its vibrant pigmentation? Dive into the research: doi.org/10.1101/2024.06.15.599

@PuckerLab @tubraunschweig

Victoria cruziana plant in the Botanical Garden at TU Braunschweig. Photo credit: Melina Sophie Nowak.
PuckerLab boosted:
Boas Puckerboas_pucker
2025-03-01

πŸš€ Ready to master ?
Today, we are diving into file handling! πŸ“ Learn how to:
βœ”οΈ Read data from a file
βœ”οΈ Write results to an output file

πŸ”— Check out the slides: github.com/bpucker/teaching

@PuckerLab @tubraunschweig

Screenshots of selected slides from #Python course. Materials are available on GitHub: https://github.com/bpucker/teaching
PuckerLab boosted:
Boas Puckerboas_pucker
2025-03-01

A new cohort of highly motivated life science students embarks on an exciting journey to explore the power of and
@PuckerLab @tubraunschweig

Screenshots of selected slides from Python course: https://github.com/bpucker/PythonTUBS
PuckerLab boosted:
Boas Puckerboas_pucker
2025-03-01

Sweet news this winter! πŸ«β„οΈ We have sequenced a cacao genome - just in time for the cozy season:

doi.org/10.1101/2024.11.23.624


@PuckerLab @tubraunschweig

Picture of the Theobroma cacao plant growing in a greenhouse in the Botanical Garden at TU Braunschweig. Figure source: https://doi.org/10.1101/2024.11.23.624982
PuckerLab boosted:
Boas Puckerboas_pucker
2025-03-01

The color difference between magenta and white flowers in Digitalis purpurea is linked to a large insertion in the DFR gene of white flowering plants, likely making DFR non-functional and blocking anthocyanin biosynthesis:
doi.org/10.1101/2024.02.14.580

@PuckerLab

Magenta and white flowers of Digitalis purpurea. Figure reference: https://doi.org/10.1101/2024.02.14.580303
PuckerLab boosted:
Boas Puckerboas_pucker
2025-03-01

Excited to share the release of our Tropaeolum majus genome sequence and annotation:
doi.org/10.1101/2024.11.01.621

@PuckerLab @tubraunschweig

Tropaeolum majus picture. Link to preprint: https://doi.org/10.1101/2024.11.01.621629
PuckerLab boosted:
Boas Puckerboas_pucker
2025-03-01

πŸš€ Exciting news for genome enthusiasts! πŸŽ‰ Our latest MGSE update now supports long reads and contains new data analyses for mapping-based genome size estimation. Curious about the details? Check it out: doi.org/10.1101/607390

@PuckerLab

Concept of MGSE. Figure reference: https://doi.org/10.1101/607390
PuckerLab boosted:
Boas Puckerboas_pucker
2025-03-01

Presenting the research of our group in front of the new TU Braunschweig biology students at Burg Warberg

tu-braunschweig.de/en/ifp/pbb
@PuckerLab

https://tu-braunschweig.de/en/ifp/pbbhttps://tu-braunschweig.de/en/ifp/pbbhttps://tu-braunschweig.de/en/ifp/pbbhttps://tu-braunschweig.de/en/ifp/pbb
PuckerLab boosted:
Boas Puckerboas_pucker
2025-03-01

Exciting news for cassava lovers! πŸ₯³ We have just released the phased genome sequence and annotation for the virus-resistant cultivar COL40. Key finding: a large region of repetitive elements on chromosome 12

πŸ”— doi.org/10.1101/2024.09.30.615

@PuckerLab @tubraunschweig

Circos plots showing the density of TEs in the two haplophases of the cassava genome sequence. Reference: https://doi.org/10.1101/2024.09.30.615795
PuckerLab boosted:
Boas Puckerboas_pucker
2025-03-01

Excited to announce the discovery of a withanolide biosynthetic gene cluster! A huge achievement from our collaboration with the Franke Lab. Explore the findings here:

doi.org/10.1101/2024.09.27.614


@tubraunschweig @PuckerLab @unihannover

Screenshot of the preprint "Phylogenomics and metabolic engineering reveal a conserved gene cluster in Solanaceae plants for withanolide biosynthesis" https://doi.org/10.1101/2024.09.27.614867
PuckerLab boosted:
Boas Puckerboas_pucker
2025-03-01

Thrilled to announce the publication of Nancy's work in PLO SONE:
Mechanisms mitigating competition between FLS and DFR
doi.org/10.1371/journal.pone.0

@PuckerLab

Competing branches of anthocyanin and flavonol biosynthesis. Figure source: https://doi.org/10.1371/journal.pone.0305837

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