Monumental paper of unnatural amino acid (UAA) mutagenesis in calcium release channels from Isabella Derler and team! Light-activated calcium release in complexes without STIM1 and tons of insights to structure function.
#GeneticCodeExpansion
Nice to see my friends Lilia Leisle, Alessio Accardi, Chris Ahern and more teaming up! Here, CLC-type ion channels, widely responsible for #chloride balance, were subjected to unnatural amino acid #mutagenesis. In a subtle example of the genre, the peptide backbone was changed at individual sites by introducing alpha-hydroxy amino acids.This showed that backbone amides coordinate permeating chloride ions.
Nice to see this work from Alberto Gonzalez and Teresa Giraldez and collaborators online! My part was small but very happy with how it has turned out. Beautiful state-dependence of crosslinks, fingerprinting movements of the BK channel.
https://www.biorxiv.org/content/10.1101/2022.12.15.520511v1.article-info
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