@posertinlab I have used #CryoSPARC's tree view since forever because it's a lot easier to understand the relationships between jobs. Even when it was slow on large projects, I put up with it because this view is so helpful. Back when I learned single-particle #cryoEM, with #RELION, I used to draw the job graph by hand as I set up new jobs, so I could remain oriented within large projects.

The performance of the tree view in CryoSPARC 4.6 is really excellent, it's a big improvement in usability compared to previous versions.
Now the only thing that could make the tree view better is an export function as I described here: https://discuss.cryosparc.com/t/job-graph-figure/15228

Reading supplementary information of papers sometimes gives nice surprises: I just noticed that two of my EMDB entries were used to train #BlushRegularization in #RELION 😮

So I could not resist the curiosity to check the list of EMDB entries used to train #ModelAngelo, but this one has none of my entries.

#CryoEM

For those that don’t know if this is good or not: Depending on the institution, above around 5.6% is abnormally high but not diagnosis threshold for diabetes. 6.5% is diagnosis threshold. Goal for a diabetic, once diagnosed, is to be below 7%. So having 6.3% means I’m achieving goal.

#Diabetes #A1c #ReliOn #CGM #GMI

I decided to compare one of those store bought A1c take home tests (ReliOn brand in this case) with a lab A1c test. After my lab blood draws, I came home and used the tester.

ReliOn: 6.5%
Lab: 6.3%

So they are reasonably accurate.

But with a CGM, my GMI was also 6.5%, so not really a good point to using a take home A1c test if you have a CGM.

#Diabetes #A1c #ReliOn #CGM #GMI

#BSI WID-SEC-2024-3257: [NEU] [mittel] #Hitachi #Energy #Relion: Schwachstelle ermöglicht Privilegieneskalation und DoS

Ein Angreifer aus einem angrenzenden Netzwerk kann eine Schwachstelle in Hitachi Energy Relion Geräten ausnutzen, um seine Privilegien zu erhöhen oder einen Denial of Service zu verursachen.

https://wid.cert-bund.de/portal/wid/securityadvisory?name=WID-SEC-2024-3257

#BSI WID-SEC-2024-2105: [NEU] [mittel] #Hitachi #Energy #Relion: Mehrere Schwachstellen ermöglichen Denial of Service

Ein Angreifer aus einem angrenzenden Netzwerk oder ein entfernter anonymer Angreifer kann mehrere Schwachstellen in Hitachi Energy Relion ausnutzen, um einen Denial of Service Angriff durchzuführen.

https://wid.cert-bund.de/portal/wid/securityadvisory?name=WID-SEC-2024-2105

New Title Alert: reliontomotools are additional tools for subtomogram analysis in Relion tomo.

Read more here: https://github.com/joton/reliontomotools

#SBGrid #SBGridsoftware #relion #tools

New Title Alert: Relion2Dynamo has been added to the SBGrid collection in February. Relion2Dynamo converts Star Files from RELION 4.0 to Dynamo Table.

More here: https://github.com/EuanPyle/relion2dynamo

#SBGrid #SBGridsoftware #RELION

Positions available at the Human Technolopole in Milan, Italy:

* Bioimage Analyst https://careers.humantechnopole.it/o/bioimage-analyst

* Research Software Engineer https://careers.humantechnopole.it/o/software-engineers

CC @florianjug

#python #java #volumeEM #ElectronMicroscopy #Relion #IMOD #CryoEM #Fedihire

I managed to find a starter kit for Accu-Chek Instant to compare with. The kit, with 100 strips, is $50. Buying more strips is around 27 cents per strip. So it's cheaper than the Guide setup, but still more expensive than the ReliOn setup by a lot.

Tempted to buy it and do more comparisons and testing.

#Diabetes #T2D #T1D #Glucometer #AccuChek #ReliOn #Comparison

Accu-Chek Guide Me starter kit costs around $60 to $90. Test strips are 40 to 50 cents per strip.

ReliOn Platinum is a rebranded Accu-Chek meter (Instant?) with Accu-Chek test strips (also Instant?). Starter kit is less than $20 and test strips are 18 cents per strip.

If you're not sure about ReliOn's reliability, but trust Accu-Chek, this is a good cheap option.

#Diabetes #T1D #T2D #Glucometer #AccuChek #ReliOn #Comparison

#OpenSoftwareAcceleratesScience! 😍
---
RT @ribo_rob
Happy to share @cryocloud_io's first blog article & benchmark! Huge thanks to @SjorsScheres for making #Relion #opensource, & @RadoDanev for uploading the data to #Empiar - this would not have been possible otherwise!
#cryoEM #cloudcomputing https://twitter.com/cryocloud_io/status/1621094105492369408
https://twitter.com/ribo_rob/status/1621094951596564480

I reprocessed the T. kivui 70S ribosomes (EMPIAR-11058) with #RELION-4 to get a ~7 Å map in situ 🤩

This map is now deposited @EMDB_EMPIAR as EMD-16451:
https://www.ebi.ac.uk/emdb/EMD-16451

#OpenSoftwareAcceleratesScience
#TeamTomo #CryoET

4. The EM grids with a drug-lead from ImmunoMet Therapeutics were tricky due to aggregation outside of a "sweet spot" of pH, protein conc and inhibitor conc. The #CRYOEM data showed a high level of heterogeneity, but were successfully separated into different states using #RELION.

And below shows the improvement in the reconstruction (coloured per local resolution) from standard #RELION (left) to Johannes' method (right).

We plan to include this functionality in #RELION-4.1, with its beta-release scheduled for mid-2023. At the moment, we are writing a paper describing the method in detail and are working hard on making the code as easy to use and install as you are used to.

@tbharat_lab is a #CryoET OG...go check out this position!

I used the data he shared from the origin #Relion subtomo paper to huge extent in developing #emClarity for my dissertation. (#ribosomes)

https://fediscience.org/@tbharat_lab/109461403525493781

Just a reminder for those struggling with #amyloid reconstruction in #relion that we recently wrote a tutorial-like paper on this in @Faraday_D, which comes with publicly available data sets at @EMDB_EMPIAR.

https://pubs.rsc.org/en/content/articlelanding/2022/FD/D2FD00034B

We are excited about the below pull request from @TandonSuyash & team at @AMD, with a #HIP implementation for #RELION, which allows running it on @AMD #GPUs! If this works well, it will become part of #RELION-4.1. 😍
#OpenSoftwareAcceleratesScience #FOSS

https://github.com/3dem/relion/pull/936/commits

Ok, let's try this out. An also on Twitter @SjorsScheres, but worried about recent developments there, and liking the #opensource and distributed nature of Mastodon. Do have a look at our own open software #RELION (relion.readthedocs.io) for #cryoEM structure determination of proteins.