#fibroblast

2025-09-03

What guides fibroblast precursors' migration from the #sclerotome during vertebrate development? This study shows that this is driven by #PDGF -mediated chemoattraction, enabling their differentiation into specialized #fibroblast subtypes @PLOSBiology plos.io/3HTZ92Y

Top: Transgenic expression of PdgfraΔK-EGFP (cyan) in nkx3.1:Gal4; UAS:NTR-mCherry (magenta) background at 56 hpf. mCherry+ EGFP+ cells are mostly restricted in the dorsal or ventral regions of the trunk. A transverse view of a 50 μm region centered on the dashed line is shown on the right, with the neural tube (nt) and notochord (n) outlined by dotted lines. Bottom: Model for how the migration of sclerotome-derived cells is regulated by chemoattraction mediated by Pdgfab/Pdgfra signaling. (A) In wild-type embryos, nkx3.1+ (cyan) sclerotome-derived cells express the receptor Pdgfra (magenta), while the medial somites express the ligand Pdgfab (orange) in response to Hh signaling. pdgfra+ sclerotome-derived cells undergo extensive migration toward both the notochord in the medial trunk and the fin fold. (B) In pdgfra−/− mutants (pdgframRFP/mRFP and pdgfraref/ref), nkx3.1+ sclerotome-derived cells fail to migrate in any direction and remain in their original location in the ventral and dorsal parts of the somites. (C) In pdgfab−/− embryos, pdgfra+ sclerotome-derived cells fail to migrate toward the notochord, although migration toward the fin fold remains unaffected. (D) In pdgfab−/− embryos with mosaic pdgfab expression, nkx3.1+ sclerotome cells can migrate toward the ectopic pdgfab-expressing cells, either medially around the notochord or ventrally toward the yolk extension.
2024-01-11

#Epithelial branching morphogenesis: @JakubSumbal @slvfre & @KoledovaZuzana use time-lapse imaging of #fibroblast co-cultures with epithelial #organoids to reveal that fibroblast contact & contractility are required for epithelial branching #PLOSBiology plos.io/48MaNog

Giuseppe MichieliGMIK69@mstdn.science
2024-01-03

-- A lower frequency of circulating B #lymphocytes by mass cytometry (CyTOF) was observed in the multidomain #deficit cluster. Circulating #fibroblast growth factor 21 (FGF21) was significantly elevated in the #mental/cognitive predominant and the multidomain clusters. Future efforts to link #PASC to acute anti-viral host responses may help to better target treatment and prevention of PASC.

2023-12-06

Does #circadian gene expression affect #reprogramming of differentiated cells? Study shows that contaminating #macrophages in #fibroblast culture can inhibit reprogramming to #neurons & #iPSCs, controlled by the clock gene Per1 #PLOSBiology plos.io/47YBh6d

2023-08-30

Lineage commitment of #dermal #fibroblast #progenitors is controlled by #Kdm6b-mediated #chromatin #demethylation
Ryan Driskell and coworkers
embopress.org/doi/full/10.1525

2023-08-24

Lineage commitment of #dermal #fibroblast #progenitors is controlled by #Kdm6b-mediated #chromatin #demethylation
Ryan Driskell and coworkers @DriskellLab
embopress.org/doi/full/10.1525

2023-08-22

Lineage commitment of #dermal #fibroblast #progenitors is controlled by #Kdm6b-mediated #chromatin #demethylation
Ryan Driskell and coworkers @DriskellLab
embopress.org/doi/full/10.1525

Francesco Baschierif_baschieri@mas.to
2023-08-17

You may have seen it on #Biorxiv but, after adding data on #Fibroblast-tracks in #3D environments (and after paying 4151 €), here is the story published in @ScienceAdvances 🎉:
Fibroblasts generate topographical cues that steer cancer cell migration

science.org/doi/10.1126/sciadv

Giuseppe MichieliGMIK69@mstdn.science
2023-02-09

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