James and Selena's paper is out in JCB!
We present a structural model of the interaction between TACC3 and ch-TOG. Then we discover Affimers that can inhibit this interaction. Expression of the Affimers in cells uncovered a new role for TACC3-ch-TOG in maintaining the pericentriolar material during mitosis.
Looks like cryo-ET studies of synapses are hot right now (pardon the pun). Another preprint, this time on dopaminergic terminals, looking at vesicle structures in situ.
and while we are at it, this other preprint uses cryo-ET on *native* synapses to look at vesicles in the terminal
https://www.biorxiv.org/content/10.1101/2024.04.01.587595v1
#structuralbiology #structbio #cellbiology
Wow! Preprint from the Rubinstein lab shows the structure of V-ATPase in synaptic vesicles. Synaptophysin is part of the V-ATPase!
We have a #PhD available!! Are you interested in #cryoEM 🔬and #RNAstructure🧬? Then this might be for you: tinyurl.com/4uecm38r It's a 4-year funded position through @BBSRCWhiteRose; DM or email me for more details. #RNA #CryoEM #molecularbio #generegulation #Structbio #structuralbiology
hmmmm, the "exlusome"
#structbio #molecularcell #Science
From: @bioRxiv_cellbiol
https://botsin.space/@bioRxiv_cellbiol/109956833473897972
Coming protein #macromolecular #crystallography #workshop in #Dubrovnik Coast (Croatia) April 16 to 21, 2023. https://htcc5.org/ 🇭🇷
Still participants slots available => do not hesitate to apply !
https://htcc5.org/2022/06/experimental-advances-in-macromolecular-crystallography/
#structbio #ccp4 #StrucuralBiology
New package! MRCFile.jl is a #JuliaLang parser for the MRC file format commonly used to store data like that produced by electron microscopy. #StructBio https://github.com/sethaxen/MRCFile.jl
I wrote this package for a project during my PhD and just now got around to registering it. I don't work with MRC files anymore, so if you do and are interested in co-maintaining, let me know!
Inhouse #structbio #xray #diffraction this afternoon. Three projects.
1) Hmm, those "spots" are very smeary, as before
2) A couple of interesting, but probably protein, spots from @xtaldave 's small crystals, so off to #DLS_MX #synchrotron they go.
3) Ohhh, better than 1.9 Å, so lets collect a quick dataset before sending off to #synchrotron.
#elonmusk may be a duchecanoe but #tesla does make some good batteries.
Unexpected power outage (no inclement weather) today, but the #structbio server stayed live!
Interesting bit here on the requirements for data storage through FAIR principles and the economic and ecological costs of long-term storage of large quantities of data.
#FAIR
#OpenScience
#datascience
#structbio
https://arxiv.org/abs/2301.01189
I have migrated my #Mastodon account to the new #structbio server:
https://struct.bio/@rdrighetto
Thanks @cryo2go for setting up the old and new servers and for the help with migration!
(also, testing cross-posting)
A quick look at the relentless pile of e-mails from during the end of year break.
You might think that the user, who was told that the function they wished to use in the crystal imaging software was broken, wouldn't try to use it on Boxing Day and then again on the New Year's BH but actually wait until being told it was fixed.
Problem is down to synchronisation issues migrating to new storage hardware so is unlikely to magically get better.
... and other techniques. My main drive is to push #cryoET in tissues to enable #structbio in #devbio systems and get the throughput with modern #cryoFIB systems needed to do #visualproteomics. Also, from a “former lifetime” still very much into #MD #QMMM #proteindesign #CompChem
