Lmst

What if we could stop DNA repair errors before they happen — by moving the damage out of danger? #DNAdamage #CancerResearch #GenomeStability

https://geekoo.news/how-protein-droplets-shield-dna-from-catastrophic-errors/

Are #autophagy-related proteins involved in #GenomeStability? This study shows that Atg11 has an essential non-canonical function in error-free #chromosome segregation via the Kar9-dependent spindle positioning pathway @PLOSBiology https://plos.io/4lfbeP1

Schematic of the findings. This study reveals two distinct pools of Atg11 in S. cerevisiae. A lower proportion of Atg11 which is less dynamic canonically localizes at the pre-autophagosomal structures (PAS) onto the vacuolar membrane, necessary for selective autophagy and dependent on actin. While the more dynamic Atg11 localizes at the SPBs in a higher proportion which is critical for high-fidelity chromosome segregation. The asymmetric Kar9 localization together with the dynamic instability of MTs is crucial for pre-anaphase spindle positioning and alignment, and chromosome biorientation. Before anaphase onset, aMTs are unstable and cyclin Clb4 levels are high. The mono-oriented kinetochores have defective tension, leading to the activation of Ipl1. Ipl1 carries out the disassembly of defective kinetochore-MT attachments via phosphorylation and activates SAC. SAC delays anaphase onset until biorientation is achieved. Upon biorientation, SAC is silenced, Pds1 gets degraded, separase is activated, and Cdc14 is released from the nucleolus via the FEAR pathway leading to proper sister chromatid separation. APC-C/Cdc20 also degrades Clb4 upon anaphase onset crucial for the stability of aMTs. Atg11 ensures asymmetric localization of both Spc72 and Kar9 at the dSPB, critical for non-random SPB inheritance.

🔬 Call for abstracts – deadline is 18 March ✨

The EMBO | EMBL Symposium 'The ageing genome: from mechanisms to disease' is back with a fresh international speaker lineup! 🧬

#EESAgeing will bring together experts in chromosome biology, ageing sciences, and disease research.

Don't miss your chance to present! Submit your abstract here: https://s.embl.org/ees25-05-ma

#ageing #DNArepair #genomestability #emblevents #telomeres #DNAdamageresponse #ageinggenome #chromatinstability #epigenetics

#ERCAdvancedGrant for Helle Ulrich of @imbmainz and @unimainz_eng: @ERC_Research grants funding worth EUR 2.5 mio. for research on #DNArepair and #GenomeStability // #LifeSciences #GenomeRegulation #ubiquitin #DNAdamage #ubiquitylation
https://nachrichten.idw-online.de/2024/06/28/erc-advanced-grant-for-helle-ulrich-for-research-on-dna-repair-and-genome-stability

Foto darf nur im Zusammenhang mit der Pressemitteilung verwendet werden.

#ERCAdvancedGrant for Helle Ulrich of @imbmainz and @unimainz_eng: @ERC_Research grants funding worth EUR 2.5 mio. for research on #DNArepair and #GenomeStability // #LifeSciences #GenomeRegulation #ubiquitin #DNAdamage #ubiquitylation
https://nachrichten.idw-online.de/2024/06/28/erc-advanced-grant-for-helle-ulrich-for-research-on-dna-repair-and-genome-stability

Discover Croatia's biomonitoring journey & compare across Europe with EDIAQI's latest findings. 🌍 Learn how factors like age, lifestyle, location, & weather affect genome damage from air pollutants. 🧬 EDIAQI sets baseline values for future research & policy development.

https://www.sciencedirect.com/science/article/abs/pii/S1383571824000251

#IAQ #EDIAQI #Biomonitoring #GenomeStability #HorizonEurope #Science

Goran Gajski

Cracking the functional domains of the RADX gene during replication. 🧬

Jessica Chevallier & Pierre Caron together highlight work from the #CortezLab which is now available online, ahead of print #JMolBiol.

Read the preLight! 👉https://prelights.biologists.com/highlights/crispr-dependent-base-editing-screens-identify-separation-of-function-mutants-of-radx-with-altered-rad51-regulatory-activity/

#CRISPR #GenomeStability #Replication #DSBs

Simplified illustration of the positive (purple) and negative (red) regulators of RAD51 nucleation (blue) on single strand DNA following replication fork reversal.

The PLK1 kinase mandates DNA polymerase theta to safeguard the #genome during #mitosis.

#preLighter Pierre Caron covers a #preprint by Camille Gelot #institut_curie and team that redefines the physiological function of #TMEJ.

#DNArepair #GenomeStability
https://prelights.biologists.com/highlights/pol%ce%b8-is-phosphorylated-by-polo-like-kinase-1-plk1-to-enable-repair-of-dna-double-strand-breaks-in-mitosis/

(A) Representative micrographs of immunofluorescence showing GFP-tagged Polϴ foci (green) localizing at mitotic DNA breaks (gammaH2A.X, Red) in human cells following replicative stress induced by the inhibition of ATR (ATRi). (B) Representative micrographs of either WT GFP-Polϴ or the GFP-PhD-Polϴ mutant in interphasic and mitotic cells. While the wild type of Poltheta (WT- Polϴ) is recruited to DNA breaks in interphase and mitosis, the mutant form that cannot be phosphorylated by PLK1 (PhD-Polϴ) is not recruited to mitotic DNA breaks.

https://igsad.de/9-phd-positions-available-in-the-research-unit-on-physiological-causes-and-consequences-of-genome-instability-1-230.html

🧬 ❌ 🧬 ❌
"Physiol. Consequences of Genome Instability"

Looking for your enthusiasm and your imagination!

📣 9 positions for PhD applicants are available within the novel research consortium FOR 5504.

Send us your application and join us in :koeln: !

Email to: schumacher-office[at]uni-koeln.de

Please re-toot! 🙏

#Science #PHDstudent #PhDposition #DNA #DNAdamage #Genome #GenomeStability
@UniKoeln
@CECAD
#KidneyResearch #Ciliopathies #Cilia #CellBiology

Highly recommend #BiomedNews http://biomed.news/ as a tool for finding papers in your field (supplementing your Twitter/Mastodon feeds of course)

I've been maintaining a report on #NucleotideMetabolism and #GenomeStability in #cancer http://biomed.news/bims-numges Usually turns up some interesting papers I missed (report is currently a little out of date though 😬 - catching up is on the to do list...)

#genomestability

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