New publication: The effects of #ecologicalinteractions and distinct conjugation rates on the invasion of a conjugative #plasmid in bacterial communities. #genetransfer
https://doi.org/10.1016/j.ecolmodel.2025.111418

DNA and jolts of electricity get people to make optimal antibodies https://arstechni.ca/eBs6 #electroporation #antibodies #medicine #Science #Biology #plasmid #Policy #DNA

Excited to kick off #IMMEMXIV in Porto, Portugal with the first session focusing on #plasmid typing for surveillance! A fantastic session thanks to our 5 great speakers and now we are going into the first debate session on strain typing in high-income vs low-resource settings. #IDSky #clinmicro

#gamers! This week's episode is a fight a long time coming, as we find out who would win between #Dishonored protagonist #corvoattano and #Bioshock protagonist #jackwynand! Outsider #Magic meets #Plasmid Powers! Wanna know who wins? Tune in now! #podcast #gaming #fyp #explorepage

How best to avoid the costs of bearing a plasmid? @rctwright @jpjhall &co find it's best for #bacteria to compensate plasmids by chromosomal (not plasmid-borne) mutations, resulting in 'hubs' for #plasmid accumulation evolution & dissemination #PLOSBiology https://plos.io/3ODbXdN

AMR Evolution, Conjugative Plasmids, Phage Therapy, and CRISPR Antimicrobials

Ilmur Jónsdóttir gave a lecture on her new phd thesis

#bacteria #amr #resistance #crispr #plasmid #phages #evolution

https://www.linkedin.com/posts/ijonsdottir_defended-my-phd-titled-evolutionary-trajectories-activity-7230187028343058434-2iCW?utm_source=share&utm_medium=member_desktop

Becoming a big fan of whole #plasmid sequencing for new constructs (Nanopore). Also a big fan of Q5 and Phusion polymerases for their accuracy and of the Hot Fusion variant of the Gibson assembly for its price and convenience (no ligation required!)

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0115318

Unveiling the Versatile World of Plasmids: Nature’s Tiny Genetic Gems

#ApplicationsOfPlasmid, #Bacteria, #BacterialPlasmid, #DNA, #Plasmid, #PlasmidFingerprinting, #PlasmidIsolation #Genetics, #MolecularBiology, #RDT In the realm of genetics, plasmids stand as miniature marvels, often overshadowed by the grandeur of chromosomes yet wielding immense…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -

https://micrordt.wordpress.com/2024/05/16/unveiling-the-versatile-world-of-plasmids-natures-tiny-genetic-gems-2/

Isolation of Plasmids from Bacterial Cells: A Step-by-Step Guide

#Bacteria, #CellLysis, #Plasmid, #PlasmidIsolation #Bacteriology Introduction Plasmids are small, circular pieces of DNA that are separate from the chromosomal DNA in bacteria. They play a…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -

https://micrordt.wordpress.com/2024/05/12/isolation-of-plasmids-from-bacterial-cells-a-step-by-step-guide/

Reproduction in Bacteria: A Fascinating World of Rapid Multiplication

#Bacteria, #BinaryFission, #Mutation, #Plasmid, #Reproduction, #ReproductionInBacteria #Bacteriology Introduction Bacteria are among the oldest and most abundant life forms on Earth. Despite their small size and simplicity, they…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -

https://micrordt.wordpress.com/2024/05/08/reproduction-in-bacteria-a-fascinating-world-of-rapid-multiplication/

Extra Chromosomal Genetic Element (Plasmid)

#Conjugation, #GeneTransfer, #GeneralizedTransduction, #Plasmid, #SpecializedTransduction, #Transduction, #Transformation #Genetics In addition to the normal DNA some bacteria also have extra chromosomal DNA (i.e. DNA other then present in chromosomes)…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -

https://micrordt.wordpress.com/2024/05/04/extra-chromosomal-genetic-element-plasmid/

Plasmid Fingerprinting

#Bacteria, #Fingerprintng, #Plasmid, #PlasmidFingerprinting #Microbiology As known, plasmid is an extra chromosomal DNA, which is capable to replicate independently within bacterial cell. Plasmid may contain…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -

https://micrordt.wordpress.com/2024/04/30/plasmid-fingerprinting/

Unveiling the Origin of Replication: A Key Feature in Plasmid Biology

#BacterialPlasmid, #OriginOfReplication, #Plasmid, #PlasmidDNA, #ReplicationOfDNA, #ReplicationOfPlasmid #Genetics Plasmids are small, circular DNA molecules found in bacteria, separate from the chromosomal DNA. They play a crucial role in bacterial genetics, often carrying genes that confer selective advantages…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -

https://micrordt.wordpress.com/2024/04/29/unveiling-the-origin-of-replication-a-key-feature-in-plasmid-biology/

Harnessing the Power of Plasmid DNA: Applications and Significance in Genetic Research

#ExpressionVectors, #Gene, #GeneCloning, #GeneEditing, #Plasmid, #PlasmidDNA #Genetics, #RDT Genetic research has revolutionized our understanding of life processes and paved the way for groundbreaking advancements in medicine, agriculture, and biotechnology.…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -

https://micrordt.wordpress.com/2024/04/28/harnessing-the-power-of-plasmid-dna-applications-and-significance-in-genetic-research/

Understanding the Art of Gene Insertion into Bacterial Plasmid DNA

#Bacteria, #Biotechnology, #ExtrachromosomalDNA, #GeneInsertion, #GeneTherapy, #Plasmid, #PlasmidDNA, #PlasmidIsolation, #PlasmidVector, #Screening, #Transformation #Genetics In the realm of genetic engineering, one of the fundamental techniques is the insertion of genes into bacterial plasmid DNA. This process, often referred to as molecular cloning, is pivotal in various fields such as biotechnology….…

https://micrordt.wordpress.com/2024/04/28/understanding-the-art-of-gene-insertion-into-bacterial-plasmid-dna/

Bacillus Anthracis

#Anthrax, #Bacteria, #Pathogen, #Plasmid #Bacteriology It was the first pathogenic bacterium to be observed under the microscope, the first communicable disease shown to be transmitted…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -

https://micrordt.wordpress.com/2024/04/24/bacillus-anthracis/

Resistance transfer factor (RTF)

#Bacteria, #Microbiology, #Plasmid, #RFactor, #RTF #Genetics This plasmid is the great choice in medical importance as it leads to the spread of multiple drug resistance among…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -

https://micrordt.wordpress.com/2024/04/22/resistance-transfer-factor-rtf/

Extrachromosomal Genetic Elements

#Bacteria, #DNA, #Microbiology, #Plasmid #Genetics Plasmids are circular DNA molecules present in the cytoplasm of bacteria, capable of autonomous replication (independent replication). Plasmids consist genes…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -

https://micrordt.wordpress.com/2024/04/22/extrachromosomal-genetic-elements/

Isolation Of Plasmid DNA By Using Alkaline Lysis

#Bacteria, #DNA, #DNAIsolation, #Lysis, #Plasmid #MolecularBiology, #RDT Plasmid is an extra chromosomal, circular, self-replicating double stranded DNA molecule found in bacterial cells. Many bacteria and few eukaryotes…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -

https://micrordt.wordpress.com/2024/04/20/isolation-of-plasmid-dna-by-using-alkaline-lysis/

More than 90% of all people in industrialised nations carry a mysterious gene sequence in their gut flora. This #plasmid could serve as a biomarker, according to a team from @HIFMB_OL & @UChicago in @CellCellPress. @UniOldenburg
https://nachrichten.idw-online.de/2024/04/16/from-a-cryptic-genetic-element-in-the-human-gut-to-a-sensitive-biomarker