If you've ever done a bit of #ProteinEngineering or #DirectedEvolution you know that funny things can happen 🤯 – and sometimes it's worth following up!
I'm excited to share that our paper is finally out in Nature Chemistry! 🎉
Read it here:
https://www.nature.com/articles/s41557-024-01490-4
An Enantioselective Biocatalytic C–H Fluorination
Directed evolution used to create a fluorinating enzyme
https://www.chemistryviews.org/an-enantioselective-biocatalytic-c-h-fluorination/
#chemistry #chemistryviews #chemiverse #research #directedevolution #biocatalysis #fluorination
Directed Evolution Used to Create Enzymes That Break Down Plastics:
Polyethylene terephthalate (PET)-depolymerizing enzyme developed using new high-throughput screening
https://www.chemistryviews.org/directed-evolution-used-to-create-enzymes-that-break-down-plastics/
#chemistry #chemistryviews #chemviews #chemiverse #research #directedevolution #breakdownplastic #PlasticPollution
Emergent biosynthetic pathway to essential amino acids by metabolic metathesis
https://www.biorxiv.org/content/10.1101/2023.09.06.556532v1?rss=1
* alkene metathesis unknown in extant biochemistry
* alkenic amino acid vinylglycine can be generated in biocompatible reaction
* vinylglycine produced in situ then used as precursor of isoleucine & methionine
* allows growth of Escherichia coli strains normally requiring these essential amino acids
#biochemistry #MolecularBiology #SyntheticBiology #DirectedEvolution #auxotrophy #prototrophy
DEVELOPING: Pfizer Disables Comments After Video Showing #DirectedEvolution
https://youtube.com/shorts/YKiihra6soE?feature=share
I think it is time to stop using #pfizer #vaccines anymore. Or any #US #vaccines for that matter
Just watch the video ...
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RT @Project_Veritas
BREAKING: @pfizer Exploring "Mutating" COVID-19 Virus For New Vaccines
"Don't tell anyone this...There is a risk...have to be very controlled to make sure this virus you mutate doesn't create something...the way that the virus started in Wuhan, to be honest."
#DirectedEvolution
https://twitter.com/Project_Veritas/status/1618405890612420609
[complaints about the content of my troeets, please send to the zoo direction]
#DirectedEvolution is a powerful method but has not been easy to do with gigantic #nonribosomal biocatalysts that make important #antibiotics. We have developed a #microfluidics screen that uses liposomes as sensors for membrane active peptides. Bacteria are grown in tiny droplets. If they make gramicidin S, the sensor liposomes burst, liberate calcein, and generate fluorescence. This was tested for screening mutants and may become a useful tool for directed evolution. https://www.biorxiv.org/content/10.1101/2023.01.13.523969v1
Knowingly.
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RT @Project_Veritas
BREAKING: @pfizer Exploring "Mutating" COVID-19 Virus For New Vaccines
"Don't tell anyone this...There is a risk...have to be very controlled to make sure this virus you mutate doesn't create something...the way that the virus started in Wuhan, to be honest."
#DirectedEvolution
https://twitter.com/Project_Veritas/status/1618405890612420609
BREAKING: Pfizer Exploring "Mutating" COVID-19 Virus For New Vaccines
"Don't tell anyone this...There is a risk...have to be very controlled to make sure this virus you mutate doesn't create something...the way that the virus started in Wuhan, to be honest."
@davidschnettler and the Hollfelder-team have used #DirectedEvolution to breed new phosphotriesterases that could be useful for bioremediation or treatment of organophosphate intoxication. Active #enzyme variants were retrieved from large libraries packaged in tiny droplets on a #microfluidics platform.
Now in #jacs
https://pubs.acs.org/doi/full/10.1021/jacs.2c10673
Here, we show that an alternative motif for phosphotriester hydrolysis exists, starting #DirectedEvolution from a metal-independent alpha/beta hydrolase which Pierre-Yves identified using functional #metagenomics:
https://www.nature.com/articles/ncomms10008
Phosphotriesters are so toxic because they irreversibly inhibit the catalytic triad of acetylcholinesterase. So, our starting point is a homologue of exactly this target, just with a cysteine instead of a serine in the catalytic triad. (3/6)
Excited to share my very first paper which was just published in #JACS:
https://pubs.acs.org/doi/10.1021/jacs.2c10673
In this work carried out during my PhD together with Jamie Klein, Tomasz Kaminski, and Pierre-Yves Colin in the Hollfelder Lab at #UniversityofCambridge we evolve a #phosphotriesterase with a new, metal-independent mechanism by using droplet #microfluidics.
Comments and feedback welcome! A thread: (1/6)
#EnzymeEngineering #ProteinEngineering #EnzymeMechanism #DirectedEvolution #Bioremediation
Hi, I'm a biochemist enthusiastic about #enzymes, #biocatalysis and #ProteinEngineering & #ProteinEvolution.
I did my PhD with Florian Hollfelder at the #UniversityofCambridge working on #DirectedEvolution of enzymes using droplet #microfluidics. Currently, I am doing a postdoc at #ETHZurich, studying which properties determine a protein’s innovability towards new function.
Looking forward to learning about cool preprints/papers and finding a lively scientific community here!
Hi everyone! I'm @basktastic on Twitter but am trying out my real name here. My #chembio #cellbio #lipidtime lab at Cornell develops tools for imaging and editing the lipidome, drawing on #bioorthogonal chemistry, #clickchemistry, #chemoproteomics, #optogenetics, #directedevolution, and more. https://baskin.wicmb.cornell.edu
