Lmst

Archiv: #Nike React Infinity Run 2 im Test. Er läuft und läuft und läuft…. https://www.sports-insider.de/nike-react-infinity-run-2-im-test-er-laeuft-und-laeuft-und-laeuft-42788/ #flyknit #NikeReactInfinityRun #Flywire #Erfahrungen #Test #Testbericht #Erfahrungsbericht #NikeReact

Archiv: #Nike #Pegasus 35 #TURBO Test. Was bringt ZoomX? Meine #Erfahrungen damit! https://www.sports-insider.de/nike-pegasus-35-turbo-test-was-bringt-zoomx-meine-erfahrungen-damit-30277/ #Erfahrungsbericht #NikeZoom #Pegasus #Test #NikeZoomX #NikePegasus #NikeReact #Testbericht #Zoom #Laufschuhtest #Flywire #Laufschuhe

Archiv: #Nike Air Zoom #Pegasus 35 im Test. #Erfahrungen mit dem Lieblingsschuh von Mo Farah https://www.sports-insider.de/nike-air-zoom-pegasus-35-im-test-erfahrungen-mit-dem-lieblingsschuh-von-mo-farah-29111/ #Erfahrungsbericht #Flywire #NikeZoom #Test #Laufschuhtest #Laufschuhe #Cushlon #Pegasus #flyknit #Testbericht

Estrenando CNCG: CNCF Valencia!
#CNCF #CLOUDNATIVEVALENCIA #FLYWIRE @julianocosta89

Juliano Costa presenting in a tv meeting room during the CLOUDNATIVEVALENCIA CNCF event at FLYWIRE

Jade Lassery speaking and Luma Levenhagen at the opening of the CLOUDNATIVEVALENCIA CNCF event at FLYWIRE

Durchbruch: Neurowissenschaftler haben erstmals den Schaltplan des gesamten Gehirns eines komplexen Tieres erstellt – dem der Fruchtfliege. #Gehirn #Hirnkarte #Hirnkartierung #Dosophila #FlyWire
https://www.scinexx.de/news/psychologie/erster-schaltplan-eines-gesamten-gehirns/

Tiny brain, big deal: fruit fly diagram could transform neuroscience;
Scientists took years to map 50m connections, which may lead to understanding of how wiring gives rise to behaviour
https://www.theguardian.com/science/2024/oct/02/fruit-fly-brain-connections-wiring-diagram-neuroscience
#news #neuroscience #science #brain #fruitfly #computerscience #connectomics #flywire

#flywire

the whorls in that recently released cubic milimeter of human brain segmentation data might be present in drosophila.

what do you all know about these spiral, condensed neural cells? has anyone assigned tissue direction vectors to voxels of segmented data and then computed the curl of that vector field as a way to find spirals?

Does anyone know what happened with the #Flywire Android App? I don't even see it in Google Play.

The connectome of the adult female fly brain, Drosophila melanogaster:

“Neuronal wiring diagram of an adult brain” by Dorkenwald et al. 2023 https://www.biorxiv.org/content/10.1101/2023.06.27.546656v1

A project that started with Davi Bock and Wei Lee developing the TEMCA for the mouse visual cortex in Clay Reid’s lab (Bock et al. 2011 https://www.nature.com/articles/nature09802), then Bock moving to #HHMIJanelia and developing the instrument much further to deliver the #FAFB volume (Zheng et al. 2018 https://www.sciencedirect.com/science/article/pii/S0092867418307876 ), and then the Seung, Murthy and Jefferis labs automatically re-registering, segmenting and annotating the whole volume via #FlyWire and more.

Extraordinary!

#Drosophila #connectomics #neuroscience #brain

Figure 1. A connectomic reconstruction of a whole fly brain. (a) All neuron morphologies reconstructed with FlyWire. All neurons in the central brain and both optic lobes were segmented and proofread. Note: image and dataset are mirror inverted relative to the native fly brain. (b) An overview of many of the FlyWire resources which are being made available. FlyWire leverages existing resources for EM imagery by (Zheng et al. 2018), synapse predictions by (Buhmann et al. 2021; Heinrich et al. 2018) and neurotransmitter predictions by (Eckstein et al. 2023). Annotations of the FlyWire dataset such as hemilineages, nerves, and hierarchical classes are established in our companion paper by Schlegel et al. (c) FlyWire uses CAVE for proofreading, data management, and analysis backend. The data can be accessed programmatically through the CAVEclient, navis and natverse (Bates et al. 2020), and through the browser in Codex, Catmaid Spaces and braincircuits.io. Static exports of the data are also available. (d) The Drosophila brain can be divided into spatially defined regions based on neuropils (Ito et al. 2014) (Ext. Data Fig. 1-1). (e) Synaptic boutons in the fly brain are often polyadic such that there are multiple postsynaptic partners per presynaptic bouton. Each link between a pre- and a postsynaptic location is a synapse. (f) Neuron tracts, trachea, neuropil, cell bodies can be readily identified from the EM data which was acquired by Zheng et al. 2018. Scale bar: 10 μm

If you're interested in my process on #Flywire proofreading #Drosophila, here's my run through video. This doesn't serve as a full newbie tutorial - but should give an idea of how I go through each cell I review.

https://www.youtube.com/watch?v=uSqxSgZDIbo

#CitizenScience

Recorded a half hour run through of my #Flywire process last night, working on getting it processed now.

This was an off the cuff recording, primarily designed to walk a partnered researcher through my process, but I'm open to making more videos about things in the future.

@brembs very intrigued by how can motor neurons and vision info communicate in this operant self learning! Wondering if #TransTango or #Flywire would shed some light?

Spent the past couple of hours untangling this set of #Drosophila neurons on #Flywire which had been improperly merged by the AI due to a misalignment in the EMs.

Misalignments are one reason humans are still needed to proofread connectomes - imaging is amazing, but not perfect!

Shown here are a TmY-type, a T4, and several T5s in the optic lobe.

A screenshot containing several 3D models of neurons of Drosophila's optic lobe, each in a different color. Shown include a TmY-type cell, and several T4 and T5 cells.

#introduction

I go by Jay, they/she.

I am the #Editor of MMOHuts and OnRPG. I have professionally written about #VideoGames since 2008.

I am passionate about #CitizenScience - my current project is mapping the connectome of #Drosophila through #Flywire. #Biology nerd.

I am also a digital artist, but I have a separate account for that: @lithiumreflections . I also enjoy #Photography.

I do my best to be #AntiRacist. I believe in boosting voices, not replacing them.

I want to share an awesome tool that #Flywire #CitizenScience has put together. Colleague and fellow CitSci "Krzysztof_Kruk" put together a "tool" that makes identifying T4 and T5 subtypes much easier.

The link contains HS (layer 1), H1 (layer 2), and VS (layer 4) neurons. By checking the terminals of a T4/T5 neuron in relation to these, you can identify them as a,b,c,d subtypes.

Details here: https://discuss.flywire.ai/t/useful-structural-patterns-in-the-optic-lobe-inferring-microstructure-from-macrostructure/114/46?u=azurejay

You have to marvel how #Drosophila has made its own clear layering system!

A screenshot of 3D renders of neurons in the Drosophila fly brain. These represent HS (white), H1 (yellow), and VS (gray) neurons in the lobula plate.

@EditorSansSerif What I find amusing about the arbor morphology of this #Drosophila neuron is how much it reminds me of neurons in #Celegans!

Do you see a left-right pair of partners?

Are there more neurons within its lineage with similar morphology?

Does it target brain areas known for compact neuropils, like compartments of the protocerebral bridge?

#FlyWire

While I'm at it, let me reach out to you wonderful #drosophila folks.

We (#Flywire citizen scientists) have come across a neuron that does not fit any types we know. Several of us have looked to see if there is an error in the trace (such as a merger) but have been unable to find any. The upper portion looks like a C-type, but it begins outside the lobula plate and goes through all three optic lobe neuropils.

Discussion link: https://discuss.flywire.ai/t/what-type-of-cell-it-is/105/317?u=azurejay

Would love insight or guidance!

A 3d model of a neuron in the drosophila optic lobe

Today I finished proofreading and classifying the outermost layer of somas in the #drosophila lobula plate cell rind through #Flywire, mapping over 270 cells!

I've broken down my findings of cell types here: https://discuss.flywire.ai/t/gallery-of-amusements-flywire-edition/34/255?u=azurejay

I'm so thrilled to have completed this first step in mapping the outer layer of lobula plate cells - all as part of #CitizenScience!

Now it's time to go another layer deep and repeat the process. :)

A screenshot of 3D models of neurons in the drosophila fly brain, showcasing over a dozen T4-type cells in the outer lobula plate area.

Need to share this great #Flywire render video!

From the video description:

"Gorgeous DPM neurons from the brain of a fly. These innervate the mushroom body.

Coming in at a combined length of 7.7 cm and looking like an elvish crown, these cells were originally identified in FlyWire by Markus Pleijzier from Gregory Jefferis lab.
Philipp Schlegel figured out that the interesting hollow tube structure actually contains fibers from the mushroom body. "

#neuroscience

https://www.youtube.com/watch?v=SuPEPAmRFiA

Almost halfway through reviewing the neurons who have somas in this single slice from the #Flywire optic lobe data set.

I'm on the very edge of the lobula plate, working on neurons with somas outside the lobula plate. The goal is to identify (and proofread as needed) cells in this region, with a special note of T4 cells.

I also am curious to see how the different cell types are arranged in the overall structure - would love to see macro patterns emerge.

#CitizenScience

#Flywire

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