Lmst

#ThreePhoton

📢 Our new study is now published in Communications Biology (Nature Portfolio):
We demonstrate deep in vivo #ThreePhoton imaging 🔬 of neurons 🧠 and glia in the medial prefrontal cortex with subcellular resolution!

👉 https://www.nature.com/articles/s42003-025-08079-8

#Neuroscience #Microscopy #CalciumImaging #Microglia #DZNE @dzne

Figure 1: Validation of three-photon imaging of mPFC in vivo.

a Setup scheme with light path and optical components. (BD: beam dump; CL: collection lens; DC: dichroic; EF: emission filter; HW: half-wave plate; M: mirror; PBS: polarizing beam splitter; SL: scan lens; TL: tube lens).
b mPFC imaging window position on the skull and representative image of the brain surface and vasculature (left; indicated ROI of z-stack shown in d); schematic of anatomical localization of imaging window relative to mPFC subareas (right).
c Confocal image of a coronal section 2 mm anterior to bregma showing YFP expression in a YFP-H transgenic mouse. Higher magnification image shows cellular YFP expression in layer V/VI of the mPFC prelimbic area. Scale bars: 1 mm, 100 µm.
d 3D reconstruction of 320 x–y frames from brain surface to 1600 µm depth at 5 µm increments (left), and selected individual frames at indicated depths (right). Scale bars: 50 µm.

© 2025 Fuhrmann, Nebeling, Musacchio et al. Published under CC BY 4.0. Source: https://www.nature.com/articles/s42003-025-08079-8

Our new #preprint on #ThreePhoton in vivo #imaging of #neurons and #glia 🔬 in the medial prefrontal cortex (#mPFC) 🧠 with sub-cellular resolution 🔍 is out! Check it out on #biorxiv:

🌍 https://www.biorxiv.org/content/10.1101/2024.08.28.610026v1

#Neuroscience #multiphoton #3p vs. #2p #twophoton #hippocampus #drosophila #MouseModel #DeepImaging #preprintalert #DZNE @dzne

Want to explore deep brain 🧠🔬 #threephoton imaging of #glioma and #microglia dynamics in the #corpuscallosum? Don't miss Felix Nebeling's (@FelixNeb) poster T24-021B at the #gliameeting in #Berlin today 💪
@dzne #GLIA2023 #3p

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