Lmst

Today's session on the 'tumor microenvironment' was filled with brillant talks by Leila Akkari (NL), Karin de Visser (NL) and Jan Böttcher (DE) at the CIMT 2024 https://www.meeting.cimt.eu/speakers-and-program
and adressed #macrophages #interleukins and #dendriticcells in #immunooncology #Immunology #Oncology @cyrilpedia @gpollara @association

@gpollara @JExpMed Intriguing. In an earlier postdoc position I had a look at #DendriticCells, and especially the #cDC1 were abundant, but seemed to be just sitting in the inflammatory environment, doing little. One of the very abundant cytokines: #IL6

We also looked at the effect of that and other #cytokines, but mostly on #Tcells, among others due to the low abundance of cDC1 in HC blood to be able to do many in vitro experiments.

"Mechanistically, glycolysis augmented ATP production to boost STING activation and STING-dependent DC antitumor functions. Moreover, DC-intrinsic STING activation accelerated HIF-1a–mediated glycolysis and established a positive feedback loop."

#Immunology #immunotherapy #DendriticCells #Metabolism

https://www.jci.org/articles/view/166031

In the middle of an excellent seminar in which Matthias Gromeier presented work on Cancer Immunotherapy with Recombinant Poliovirus, the Zoom session was ended and no luck from the organization's side to restart. 😭

The first 10-15min showed work linking back to original work and ideas from Albert Sabin and David Bodian. Specific targeting of #DendriticCells and #Macrophages, #interferon response.

Hopefully they recorded the seminar.

#cancer #immunotherapy

The paper focuses on #DendriticCells (DC) and #monocytes from a local inflammatory site, in this case synovial fluid from swollen joints of mainly juvenile #arthritis patients.
Very short: one type of DC were very much enriched but seemed relatively quiescent despite the inflammatory surroundings, while other DC and monocytes behaved clearly pro-inflammatory.

Last week, the paper based on my work in the Wilhelmina Children's Hospital (WKZ) was published, with the title "Conventional dendritic cells type 1 are strongly enriched, quiescent and relatively tolerogenic in local inflammatory arthritis".
https://doi.org/10.3389/fimmu.2022.1101999

Credits to Anoushka Samat for taking care of this after I left the department. Without her this work would not be out there. And of course thanks to the rest of the team.

#immunology #RNAseq #inflammation #DendriticCells #monocytes

Check our last contribution to study #CellMigration in #DendriticCells, by #LolaH and @LpzLpz_Tami
(Thanks to @renkawitzteam for the invitation)

There is a lot more assays to have fun !

Full @EurJImmunol: https://onlinelibrary.wiley.com/doi/full/10.1002/eji.202249925

Here goes the #dendriticcells merrily via #lymphatics taking antigens - the presents for #Tcells . Will they take them? What will they do? Best xmas card from #CellCartoons hope she comes to mastodon soon!

Y’all, this thread by @jeffgilchrist is so important and good. All about #Covid and our emerging understanding of its deleterious effects on #immunity.

#immunesystem #Tcells #dendriticcells #naiveTcells

https://mstdn.science/@jeffgilchrist/109558435801043562

Dear people interested in #organoids #Tcells #Macrophages #Bcells #DendriticCells #MucosalImmunology #microbiome #OrganOnChip #MicroPhysiologicalSystems #IBD #CeliacDisease #iPSCs,

where are you? I want to follow you!

Best regards,
Me

'This article (...) provides detailed protocols for the preparation of single-cell suspensions from various mouse nonlymphoid tissues, including skin, intestine, lung, kidney, mammary glands, oral mucosa and transplantable tumors, and instructions for multiparameter flow cytometry analysis of DC subsets.'

#Immunology #DendriticCells #FACS #FlowCytometry

https://onlinelibrary.wiley.com/journal/15214141