A #RaspberryPi-#automated #OpenSource milligram-scale, four channel #protein #purification system based on liquid #chromatography:

-#GUI & #Python API

https://doi.org/10.1101/2023.08.09.552685
#DIYbio #lab #instruments #LC #FPLC

#Colosseum: A <$100, #3Dprinted, scalable & #Arduino-#automated #OpenSource fraction collector:

Paper: https://doi.org/10.1016/j.ohx.2021.e00201
YouTube: https://www.youtube.com/watch?v=yG7ECh5GO0o
Build instructions: https://doi.org/10.5281/zenodo.4677604

#DIYbio #lab #instruments #fluidics #LC #HPLC #FPLC #chromatography

Found a really nice #opamp to design my #UVC detector for my #bespoke #FPLC project. It has really low input bias current and offset #voltage. It's a bit slow with a slew rate of about 0.35v/us (would take about 14 #microseconds to rise from 0 to 5v) but, for my needs, this is perfect. Also found a better #UVC detector that has high sensitivity around 280nm. Time to draft a #transimpedence amplifier #circuit #PCB!

I wrote up today's findings in my research blog. Made a simple desalting column out of parts from McMaster which looks promising. All parts are hotlinked in the post so feel free to try your hand as well. Lots of room for improvement. Enjoy!

#FPLC #chromatography #diybio #protein #desalting #column #handmade #bespoke

https://www.atinygreencell.com

Conductivity module prototype for my crappy bespoke FPLC coming along nicely...

#fplc #diybio #conductivity #protein #chromatography

Fused silica tubing for UV detector of my crappy bespoke FPLC. Gonna wait for silicone to set, add another coat to reinforce the connection, and then 3D print a holder for the UVC sensor and 280nm LED. Gonna make a constant current driver later...

#fplc #diybio #laboratoryHardware #bespoke #handmade #protein #UVC #ultraviolet #3dprinting