Lmst

My pombe (fission yeast) didn’t mate 😌
Preparing a different medium for mating, giving them some B vitamins 😉
Yeast needs vitamins, I also need vitamins 🤔

#Pombe #Yeast #Science #Experiment #Vitamins #Health

Biotin, pantothenic acid, nicotinic acid, and inositol near a scale.

A beautiful Western blot that I did with new undergraduate students 🥳

S. pombe nmt1 (81) promoter worked well to repress gene expression 🧐

#Science #WesterBlotting #Protein #Pombe #Promoter #Photography

A PVDF membrane with protein bands detected by antibodies and chemiluminescent substrate.

I spent the day running experiments with yeast (S. pombe) instead of sitting in front of a computer 😊
I wondered how much yeast (S. cerevisiae) is in this cloudy IPA, looks like way more than I used in my lab today 🤔

#Beer #IPA #Yeast #Pombe #Science #Photography

Cloudy golden IPA in a pint glass with its can on the side. Iron Hill Brewery, Philly Phavorite IPA.

#FlocculationFriday

A few S. pombe strains don’t flocculate 🤔
My favorite mutant doesn’t seem to be great for brewing beer 🤔
My research doesn’t have anything to do with brewing, though 🤔

#Yeast #Pombe #Beer #Science #Philadelphia #Flocculation #Photography

Eight test tubes with yeast culture on a green rack. Seven cultures have flocculation, while three don’t.

Tomorrow is the deadline, the office staff told me that the NIH grant application system has been a bit unstable, my brain is also a bit unstable because of sleep deprivation, so I made some agar plates to clear my mind, it seemed to help 🤔

#Grant #Proposal #Science #MentalHealth #Writing #Agar #Yeast #Pombe

Three stacks of Petri dishes containing agar growth medium sit on the black research bench, with bottles and tubes of buffer and chemicals and a green tube rack in the background.

#ThursdayThoughts

While running last-minute experiments to support my proposal, a student in a neighboring lab said he’d never seen me “doing science” before. I'm sure he meant “doing experiments,” but I do feel I'm “doing science” when I'm doing experiments, rather than working (on science) at a computer🤔

#Science #Yeast #Pombe

An open 96-well plate, a few closed Petri dishes, an 8-channel pipette are in a black research bench. A metal 48-pin replicator is sitting on the 96-well plate.

Belated introduction after more than two years on Mastodon 😊
While I post a lot about #Nature, #River, #FlyFishing and #IPA, I use the fission yeast Schizosaccharomyces pombe for my research. At some point, I plan to brew #Beer🍺

#FissionYeast #Pombe #Science #SchizosaccharomycesPombe

A Petri dish with agar growth medium has fission yeast colonies from tetrads. Tetrad colonies are aligned vertically.

A Petri dish with agar growth medium has patches of fission yeast that are white or pink.

Got some pink yeast 😊

Can we make pink beer if we use this yeast strain ??? 🤔

The ade6 gene mutation causing red pigments for this color change in the fission yeast S. pombe 😉

#Pombe #FissionYeast #Yeast #Science #Beer #Pink

An alignment of patches of fission yeast growth on an agar medium on a Petri dish.
Some show pink, some are white.

#TetradTuesday

Did some tetrad dissection of fission yeast (S. pombe) spores on Friday 😊
Today, they are showing pretty colonies 🥳

Tetrad = four spores produced after yeast meiosis

#TetradDissection #Tetrad #Spore #Yeast #FissionYeast #Science #Pombe

S. Pombe tetrads from the parental asci are order aligned and have formed colonies on agar growth medium in a petri dish. There are some missing colonies when spores are not viable to grow and proliferate.

Did some yeast (S. pombe) tetrad dissection 😉
It’s pretty meditating on Friday night after Happy Hour ☺️
Hoping to get some nice results 😊

#Yeast #Pombe #Tetrad #Science #Genetics #Meditation #HappyHour

A microscope with a micro manipulator that has a needle to dissect yeast tetrads. There is a Petri dish with agar growth medium on the stage. The Petri dish is placed upside down, so that yeast spore can be manipulated from the bottom by the needle.

Making some agar growth medium for facilitating fission yeast mating😊
They have two mating types: h- and h+ 😉
Malt extract in the medium 😊
Fission yeast - Schizosaccharomyces pombe 😉

I just made a successful pollination for our pumpkin (I think), now fission yeast’s turn 😊

#Experiment #Science #Pombe #Yeast #Agar #Mating #MatingType #Pumpkin

Two stacks of Petri dishes with agar growth medium are on a black-top research station. A flask and its cap are on the side.

It's been a while since I last grew yeast (Schizosaccharomyces pombe) myself - exciting 🤩
A colleague asked, "Are you doing experiments?" and I replied, "Yes, just a little." 😉
It’s also helping me boost my step count, definitely better than sitting in my office all day 😊

#Experiment #Science #Pombe #Yeast #Agar

Two stacks of plates are incubating in an incubator with a silver lining, alongside several bottles of chemicals arranged nearby.

Schizosaccharomyces pombe on the cover of JCS - beautiful as usual 😊
My favorite yeast 😉

#Yeast #Pombe

https://journals.biologists.com/jcs/issue/137/18

What exactly is the point of beer🍻pombe being put into glass bottles🤐?

Glass bottles are commonly used to store & sell variety of beverages, wine, beer, spirits and even sparkling water. So, #HapaUjanjaTu remember why WHY👆👆👆

Remember, there's always more nonsense here👇👇

www.5021.tips

👆👆👆
You may want wast some of your time there👆
#5021tips #beerbottles #alcoholmarkers #Alcohol #beer #howitsmade #glassbottles #beerbottle #pombe #inzoga #jifunze #KnowItAll #BeInformed #jifunze #ujanja

A postdoctoral position is available in the lab of Henry Levin in the Eunice Kennedy Shriver National Institute of Child Health and Human Development to study the rDNA repeats of Schizosaccharomyces #pombe.

https://community.alliancegenome.org/t/sequence-variation-and-copy-number-control-of-rdna-repeats/7424

#yeast #rDNA #job #postdoc

#pombe #pombase

Abstract

Standardized nomenclature for genes, gene products, and isoforms is crucial to prevent ambiguity and enable clear communication of scientific data, facilitating efficient biocuration and data sharing. Standardized genotype nomenclature, which describes alleles present in a specific strain that differ from those in the wild-type reference strain, is equally essential to maximize research impact and ensure that results linking genotypes to phenotypes are Findable, Accessible, Interoperable, and Reusable (FAIR). In this publication, we extend the fission yeast clade gene nomenclature guidelines to support the curation efforts at PomBase (www.pombase.org), the Schizosaccharomyces pombe Model Organism Database. This update introduces nomenclature guidelines for noncoding RNA genes, following those set forth by the Human Genome Organisation Gene Nomenclature Committee. Additionally, we provide a significant update to the allele and genotype nomenclature guidelines originally published in 1987, to standardize the diverse range of genetic modifications enabled by the fission yeast genetic toolbox. These updated guidelines reflect a community consensus between numerous fission yeast researchers. Adoption of these rules will improve consistency in gene and genotype nomenclature, and facilitate machine-readability and automated entity recognition of fission yeast genes and alleles in publications or datasets.

@alameth
I also have the yeast hashtag - especially - pombe 😊 but I don’t know much about yeast strains for beer 🤔 I use yeast for my other part of my life - some day, I will try pombe beer 😊 #pombe

Pombe 2023, Hiroshima, Japan 😀

The 11th Fission Yeast International Meeting

#science #pombe #yeast #conference #hiroshima #japan

Note for our DNA snack 😀

We investigate the function of a gene called “maf1” 🧬
This licorice-gummy is based on the DNA sequence of this gene from the brewer’s yeast Saccharomyces cerevisiae 😀 We also checked maf1 genes from the humans and also from the fisison yeast Schizosaccharomyce pombe, which we love and is used to brew pombe beer 🤔 But the best coloring was obtained from S. cerevisiae, so here it is (revised version) 😋

#pombe #cerevisiae #yeast #beer #science #gene #art #deco

#pombe

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