Lmst

Androgen receptor-positive #ProstateCancer cells express hormone-dependent tRNA halves, but what are they for? This study shows that the 5′-tRNALysCUU half promotes #CellCycle progression by destabilizing the mRNA of p21, a negative regulator @PLOSBiology https://plos.io/3SHIilO

Schematic representation of how the 5′-tRNALysCUU half promotes cell proliferation. Lefthand panel shows the normal situation in AR-dependent prostate cancer cells, with the 5'-tRNA half destabilising p21 mRNA, resulting in lower p21 levels and boosted cell cycle progression. Righthand panel shows cells with reduced levels of 5'-tRNA halves, where the higher levels of p21 result in cell cycle arrect.

What is the role of m5C #RNAmodifications during neural development? This study shows that m5C reader protein Ybx1 plays a crucial role in promoting #CellCycle progression of embryonic cortical #ProgenitorCells, essential for proper cortical development @PLOSBiology https://plos.io/3SUN4we

Left: Coronal brain sections of control (top), heterozygous (middle) and knockout (bottom) mice at E13.5 were stained with antibodies recognizing BrdU and Ki67. Pregnant mothers received a BrdU pulse 24 h before embryo collection. Representative cortical regions were shown. Scale bar, 50 μm. Right: A model in which the m5C reader protein Ybx1 regulates the stability of transcripts of key cell cycle regulatory proteins, and its loss leads to impaired proliferation and differentiation in cortical progenitor cells. Image credit: Jian Zhang

Multiple #myeloma often requires IRE1 to sustain malignant growth, but why? This study shows that IRE1 promotes #CellCycle progression via the transcription factor IRF4, paving the way for new therapeutic strategies @PLOSBiology https://plos.io/43TnG0L

Hypothetical model for nonenzymatic IRE1 cell cycle regulation via IRF4. Schematic representation of IRE1 supporting IRF4 activity/expression, promoting E2F1 and CDC25A transcription, in turn driving CDK2 activity to enable unconstrained cell-cycle progression. Silencing of IRE1 increases IRF4 phosphorylation on Ser114 and Ser270, which attenuates IRF4’s self-sustaining transcriptional activity, depleting E2F1 and CDC25A and leading to cell-cycle arrest. Phosphoresidues annotated in red: inhibitory. Phosphoresidues annotated in green: activating. Created in BioRender.

ER-resident IRE1 plays a role in #cancer development via enzymatic activation of XPB1 TFs and regulated #RNAdecay. This study shows that some cancer cells also have an unexpected nonenzymatic dependency on IRE1 to promote #CellCycle progression & tumor growth @PLOSBiology https://plos.io/3EaNFX4

Detection of endogenous Halo-tagged IRE1 and non-tagged Lamin B1 by confocal microscopy. U2OS HaloTag cells, containing a C-terminal HaloTag into the endogenous IRE1 gene locus, were examined. Cells were cultured in the presence of Janelia 646 HaloTag ligand (green), fixed, stained with anti-Lamin-B1 (red) antibodies, and analyzed by confocal microscopy. Left: Merge field of IRE1 (red), Lamin B1 (green), and nucleus (blue) is shown. Scale bar = 5 μm. Right: Close up of the nuclear envelope where each channel is shown separately and merged. Scale bar = 2 μm. Pixel size = 48 nm. Representative image of two independent experiments shown.

Current methods to study #CellCycle progression in #yeast ignore variations in cell size. The FLCCR reporter system can quantify the duration of cell cycle phase in single cells, allowing independent assessment of cell size & cell cycle progression #plosbiology https://plos.io/4j48NxJ

From post 5,
Amino acids that are enriched/depleted next to the phospho-site reflect which protein kinase enzymes did the phosphorylation.

Phospho-dawn sees acid-directed sites being phosphorylated (D and E enriched at ZT0),
but by evening it's #CellCycle kinases guided by Proline (P at ZT12-16). 😎

#Shiftwork

Motif plot showing which amino acids are enriched or depleted in the residues next to the phosphorylated Serine or Threonine, which reflects the preferences of the protein kinase enzymes that do the phosphorylation. Those enzymes seem to work in alternate shifts, because acid residues (D/E) flank the rhythmic phospho-dawn sites that are most phosphorylated at ZT0 before dawn. Many of the evening-peaking sites are followed by Proline (P), characteristic of GSK3b and the cell-cycle kinases. (from Figure 5C)

Apicomplexan #parasite division: @MGissot &co show that the #phosphatase TgPP1 in #Toxoplasma regulates the #CellCycle & amylopectin accumulation by dephosphorylating inner membrane complex & starch-binding domain proteins #PLOSBiology https://plos.io/3XwMYgj

How do cells respond to defects in nuclear reassembly after #mitosis? This study in #Drosophila shows that damaged cells are either removed by #apoptosis or activate a p53-dependent #CellCycle checkpoint to maintain tissue integrity #PLOSBiology https://plos.io/3AA4g4c

“In this preprint, the authors (Saykali et al.) turned into cinematographers, capturing the real time dynamics of CDKs using a very sensitive sensor.” 🎬 🎥

Another lovely, entertaining preLight prepared by Mansi - check it out! ⬇️ 👀

#preLight 👉 https://prelights.biologists.com/highlights/lineage-specific-cdk-activity-dynamics-characterize-early-mammalian-development/

#development #biology #preprint #cellcycle #imaging

Quantifying DNA replication speeds in single cells by scEdU-seq #singlecell #cellcycle #newtech

https://www.nature.com/articles/s41592-024-02308-4?utm_source=nmeth_etoc&utm_medium=email&utm_campaign=toc_41592_21_7&utm_content=20240713

Understanding Meiosis: The Intricacies of Eukaryotic Cell Division

#Cell, #CellCycle, #CellDivision, #EggCell, #GameteFormation, #GerminativeLayer, #HaploidCell, #Meiosis, #SpermCell #CellBiology In the intricate dance of life, cell division plays a pivotal role in the perpetuation of species and the generation…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -

https://micrordt.wordpress.com/2024/05/16/understanding-meiosis-the-intricacies-of-eukaryotic-cell-division/

Unraveling the Mechanisms: Regulation of DNA Replication in Bacterial Cells

#Bacteria, #CellCycle, #DNA, #DNAReplication, #DnaA, #OriC, #RegulatoryProteins, #Replication #Genetics, #MolecularBiology DNA replication is a fundamental process in all living organisms, ensuring the faithful transmission of genetic information from one generation to the next. In bacterial cells, where simplicity meets efficiency…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs…

https://micrordt.wordpress.com/2024/05/01/unraveling-the-mechanisms-regulation-of-dna-replication-in-bacterial-cells/

The Procaryotic Cell Cycle

#Bacteria, #CellCycle, #CellDivision, #DNA #CellBiology The procaryotic cells are considered as primitive cells. It is because of its simple structure and limited functionalities. It is…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -

https://micrordt.wordpress.com/2024/04/29/the-procaryotic-cell-cycle/

The Nucleus And Cell Division

#CellCycle, #CellDivision, #Meiosis, #Mitosis #CellBiology Nucleus is a membrane bound and most visually prominent organelle in eukaryotic cells. Nucleus is exclusively present in eukaryotic cells…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -

https://micrordt.wordpress.com/2024/04/28/the-nucleus-and-cell-division/

Cell cycle genes are re-activated in some #neurons as our brains age. This study uses bioinformatics to characterize the prevalence, nature & fate of these #CellCycle re-entry neurons, within the healthy #aging & diseased human brain. #PLOSBiology https://plos.io/4dbHoXt

When you have “ATM” in your name your destiny is to either work for a bank or become a researcher specialising in #DNAdamage #science #CellCycle

https://pubmed.ncbi.nlm.nih.gov/38625790/?utm_source=Feedly&utm_medium=rss&utm_campaign=None&utm_content=1TkPOxkdaxtnnrl5wnZzn2VRNhPyzrYC_QUuJ0PP1Pdf91LMB7&fc=None&ff=20240416205101&v=2.18.0.post9+e462414

Picture showing authors of a research paper. The senior author has initals ATM

New #ReviewedPreprint investigates the regulatory role of Mecp2 in #quiescence exit, providing “fundamental insights” into the mechanism controlling the #CellCycle.

#Preprint with public reviews by @eLife.

https://sciety.org/articles/activity/10.1101/2023.07.24.550263?utm_source=mastodon&utm_medium=social&utm_campaign=sci_mastodon_2023_190

A quote from the preprint review written in grey on white background with orange triangles in two corners of the graphic. The quote reads: Conceptually, this is an interesting study with very well-executed experiments and controls. eLife reviewers about Yang et al. (2023).

Chromatin is less mobile during S-phase compared to G1 and G2 phases of the #CellCycle, new study finds.

#ReviewedPreprint with public reviews by @eLife-

https://sciety.org/articles/activity/10.1101/2023.03.07.531331?utm_source=mastodon&utm_medium=social&utm_campaign=sci_mastodon_2023_177

Sensitization of cancer cells to ferroptosis coincident with cell cycle arrest by @JasonRodencal, Scott Dixon @SJD956, and colleagues at @Stanford and others #ferroptosis #cellcycle #cancer https://www.cell.com/cell-chemical-biology/fulltext/S2451-9456(23)00372-0?rss=yes&utm_source=dlvr.it&utm_medium=mastodon

Proliferating cells need to maintain a stable size distribution across the generations. This study shows that this is achieved by tightly controlling #CellSize variation throughout the #CellCycle, mainly via regulation of cell growth rate #PLOSBiology https://plos.io/4aMUWHS

#CellCycle

Client Info