Genotyping by sequencing (Genetics 🧬)
In the field of genetic sequencing, genotyping by sequencing, also called GBS, is a method to discover single nucleotide polymorphisms in order to perform genotyping studies, such as genome-wide association studies. GBS uses restriction enzymes to reduce genome complexity and genotype multiple DNA samples. After digestion, PCR is perfo...
https://en.wikipedia.org/wiki/Genotyping_by_sequencing
#GenotypingBySequencing #Genetics #Biotechnology #DnaSequencing
"After more than two decades of work, researchers have achieved a genetics milestone: they have successfully sequenced the complete genomes of six ape species, a feat that seemed impossible just a few years ago."
Next Generation Sequencing: A Brief Guide to NGS Technology!
Explore how Next-Generation Sequencing (NGS) is evolving genomics. Get to know about the key benefits of NGS technology in medicine.
Keep Reading On Here: https://rb.gy/n3m362
#genetictesting #nextgenerationsequencing #generationsequencing #dnasequencing #healthcare
Posted on #Aseq #NGS #DNASequencing #Illumina #PacBio
Genotype (Genetics 🧬)
The genotype of an organism is its complete set of genetic material. Genotype can also be used to refer to the alleles or variants an individual carries in a particular gene or genetic location. The number of alleles an individual can have in a specific gene depends on the number of copies of each chromosome found in that species, also referred to as ploidy. In diploid species like...
The Register: DNA sequencers found running ancient BIOS, posing risk to clinical research . “Cybersecurity shop Eclypsium claims security issues affecting leading DNA sequencing devices could lead to disruptions in crucial clinical research. The iSeq 100, developed by manufacturer Illumina, was torn down and found to be running an insecure BIOS implementation that opened up the device to […]
A California man’s conviction despite DNA evidence pointing to his innocence has reignited debate over forensic algorithms.
Critics question the transparency and reliability of tools used in probabilistic genotyping like TrueAllele.
https://undark.org/2024/12/16/unleashed-data-dragnet/
#criminaljustice #science #DNAsequencing #crime #Algorithms #undark #unitedstates #USPolitics #usa #us #biology #genotyping #dna #california
Can we please have more of such #ecology #zoology project videos? 😉
Here they are combining #research on #arthropods in mountain areas, #ElevationalGradients, and #DNAsequencing to assess the #SoilFauna in 🇿🇦, old and new one.
One of the findings from the paper:
Expansion of our knowledge about the evolution of Orchids
https://phys.org/news/2024-02-orchid-family-emerged-northern-hemisphere.html
#orchid #genome #TreeOfLife #DNASequencing
The old #Veritasium video about "How They Caught The Golden State Killer" https://youtu.be/KT18KJouHWg raises some important points: The genetic information stored in various databases allows identifying distantly related individuals. Most are comfortable with that idea, as long as we're catching a killer or sexual predator. But the legal frameworks that control the law enforcement access to these database are not fixed and the laws governing the access can change. Let's image that our ability to identify certain criminal tendencies from genome gets to the point we can identify potential predators or future killers even before they potentially commit these acts. It might be difficult to argue against employing such capability to pre-emptively stop crime. Going this path is an insanely slippery slope, and unfortunately much of it is out of our control anyway. So many people have already deposited their genetic material to these databases that regardless how you personally feel about it, we're all already in the databases via our relatives. Since the laws will be enacted by politicians voted to the office by the majority, individual objections will carry little weight. To make matters worse this is a highly complicated topic which is easy to twist by using populist catchphrases. I'm no pessimist by any measure but I find this moderately terrifying. #thoughtoftheday #genetics #dnasequencing #profiling #privacy
Using #DNAsequencing to show how marine worms adapt to #DeepSea conditions https://phys.org/news/2023-10-dna-sequencing-marine-worms-deep-sea.html
Third-Generation #Sequencing Reveals the Adaptive Role of the #Epigenome in Three Deep-Sea #Polychaetes: Maeva Perez et al. https://academic.oup.com/mbe/article/40/8/msad172/7231443
"#epigenetic regulation by #DNAmethylation could be an adaptive mechanism that counteracts the effects of hydrostatic pressure at the bottom of the #ocean"
Using #DNAsequencing to show how marine #worms adapt to deep-sea conditions https://phys.org/news/2023-10-dna-sequencing-marine-worms-deep-sea.html
Third-Generation #Sequencing Reveals the Adaptive Role of the #Epigenome in Three #DeepSea #Polychaetes: Maeva Perez et al. https://academic.oup.com/mbe/article/40/8/msad172/7231443
"#Methylation is an #epigenetic process that chemically modifies some of DNA bases. It plays a role in environmental acclimatization, making organisms living in habitats where conditions are unpredictable more resilient to environmental changes"
Today's topic is "Next-Generation Sequencing" 🧬 🤩 🧬
Our graduate students(MCBG/Biochemistry/Cancer Bio/Biotech) leaning and discussing sequencing analyses led by Dr. Joshua Mell, Drexel University College of Medicine 🤔
#BiomedicalScience #GraduateSchool #GraduateStudies #NextGenSequencing
#DNASequencing
#RNASequencing
Human Y chromosome fully sequenced
https://www.newscientist.com/article/2388720-the-human-y-chromosome-has-been-fully-sequenced-for-the-first-time
Discussion: https://news.ycombinator.com/item?id=37256817
* 20y after Human Genome Project declared complete, Y chromosome fully sequenced for 1st time
* large amt. repetitive DNA i.e. difficult to compile complete sequence
* most people have 22 pairs of chromosomes + 2 sex chrom.: pair of X, or 1 ea. X, Y
#genomics #Ychromosome #HumanGenome #HumanGenomeProject #NGS #CellularBiology #bioinformatics #RepetitiveDNA #DNAsequencing #DNA #genomics
AAAS: "Air pollution sensors can double as biodiversity monitors." Study demonstrates an inexpensive way to sniff out species from 'environmental' or eDNA snagged in air filters. “We are starting to see [that] eDNA is left everywhere,” says Kristine Bohmann, a molecular ecologist at the University of Copenhagen. 'Larger animals slough off DNA in skin, scales, and feathers, and researchers have also detected eDNA from mussels, sponges, dung beetles, spiderwebs, and even carnivorous plants, on land and in water.' Bohman + other research teams have also shown they can find DNA from plants, insects + mammals. "Samples included DNA from threatened species such as hedgehogs and songbirds; ash, linden, and oak trees; and crops such as wheat and cabbage." If old filters are kept dry, the DNA is stable. Per Stenberg, a geneticist at Umeå University 'examined DNA from 30 years’ worth of archived air filters, removed weekly from sensors set up to detect radioactive fallout from nuclear explosions across Sweden.' In theory, documentation of biodiversity could prompt greater efforts to maintain it. Lots of intriguing possibilities. Some a bit worrisome. #dnasequencing #monitoringsystem #biodiversity
new citation: `DNA Detection Using a Single-Layer Phosphorene Nanopore` https://pubs.acs.org/doi/10.1021/acsanm.3c00937
#singleMolecule #biosensor #nanopore #DNA #sequencing #DNAsequencing
The UK #research project "The Respiratory #Virus and Microbiome Initiative" could be used to monitor #viral changes in the future. Using a single nasal swab sample, #DNASequencing should enable identification of all viral, bacterial and mycotic species. The goal is to bring a low-cost and easy-to-use #technology to the market, yet achieve a broad spectrum of testing.
This is my #Introduction post!
Once per day, I generate a random #DNA sequence position weight matrix and draw a sequence logo based on it.
Please follow, favorite, and boost if you like DNA logos, #bioinformatics, #genomics, or just #MolecularBiology in general!
#Molecular #Biology #MolecularBio #DNASequence #DNASequencing
Genome Editing Procedures Optimized:
Scientists succeed in boosting the efficiency of CRISPR/Cas9 and related methods and modifying initially inaccessible DNA sequences. They fine-tuned these tools to enable effective genetic screening for modelling specific gene mutations
https://elifesciences.org/articles/72124
https://elifesciences.org/articles/70558
#crispr #genetics #immunology #molecularbiology #genomeediting #dnasequencing #dna #cellbiology
When I started #DNAsequencing, I was manually reading A,C,G and T as individual bands on an autoradiograph of a sequencing gel (#SangerSequencing)...
Now this new tool offers a universal sequencing read interpreter...
New research in Science Advances on:
