Lmst

Amplicon #sequencing of pasteurized retail #dairy enables genomic #surveillance of #H5N1 avian #influenza virus in #USA #cattle, https://etidiohnew.blogspot.com/2025/06/amplicon-sequencing-of-pasteurized.html

SEQUENCING (Ucraïna) presenta nou single: "Втрачена земля" #Sequencing #MelodicBlackMetal #Juny2025 #Ucraïna #NouSingle #Metall #Metal #MúsicaMetal #MetalMusic

Our poster abstract for #ASMicrobe2025 is up! First bit of info from our warehouse lab led Haloarchaea study project. Session EEB-P-302 - EEB02 Saturday Microbial Evolution. Please feel free to come say hi 👋

#microbiology #archaea #sequencing #methylation

https://www.abstractsonline.com/pp8/#!/20974/presentation/3670

1 of 2 posters for the summer picked up! I'll be presenting this one at upcoming Boston Bacterial Meeting #BBM2025.

A specific type of plasmid replication gene (RPA) is overreprented among Deinococcota & analysis hints at introduction of the MGE early in its history, tracing back to Allomeiothermus #microbiology #amateurbiology #sequencing

Since this is polishing up of such an older data, I'd say it's the most amateur-like (in a good way) of all stuff coming out of our warehouse lab right now

Poster "RPA plasmid replication mechanism is conserved across members of phylum Deinococcota, suggesting origin from a common ancestor"

Kurze Erinnerung: Noch könnt ihr euch für ein Gespräch mit den DSMZ-Forschenden zu den diesen Themen anmelden 👇

Anmeldung: https://www.leibniz-gemeinschaft.de/ueber-uns/neues/veranstaltungen/book-a-scientist

#BookAScientist #ernährung #pflanzengesundheit #pflanzenviren #phagen #antibiotika #gesundheit #cassava #sequencing

Wondering how plant virus detectives work? Ask our expert Paolo Margaria at #BookAScientist.
appointments: https://www.leibniz-gemeinschaft.de/ueber-uns/neues/veranstaltungen/book-a-scientist

#sequencing #plantvirus #hightroughput

SciTech Chronicles.. . . . .May 13th, 2025

https://bit.ly/stc051325

#AEM #PEM #Alkaline #nickel #pozzolana #"lime clasts" #quicklime #"hot mixing" #wastewater #"genetic material" #sequencing #PCR #genomes #CCR5 #"1000 Genomes Project" #chemokines #industrial #enol #light-powered #separation

ONT is finally fully discontinuing the original MinION Mk1B model (the original silver stick we're all familiar with). End of en era. I hope at least some future flow cells will continue to be compatible with the older platform 😔

#microbiology #sequencing #bioinformatics

#Wastewater #sequencing reveals the #genomic landscape of #Influenza A virus in #Switzerland, https://etidiohnew.blogspot.com/2025/04/wastewater-sequencing-reveals-genomic.html

Whole genome #sequencing has become cheap enough to become a routine early diagnostic test for #rareDiseases. This can help patients to avoid years of fruitless diagnostic odyssey. Could also facilitate development of cures. Moving case report. https://www.nature.com/articles/d41586-025-01014-1

#Molecular #epidemiology of #Kyasanur Forest Disease employing ONT-NGS a field forward #sequencing, https://etidiohnew.blogspot.com/2025/03/molecular-epidemiology-of-kyasanur.html

What do #nanopore #sequencing #bioinformatics folks think about this one? New preprint on hashing raw nanopore signals and using them directly for de-novo assembly.

https://arxiv.org/abs/2503.02997v1

Hypothetical blow5-to-assembly pipe would be interesting, though figuring it out is way out of my depth.

Many published papers are reports on #large-scale results, with interesting conclusions and predictions but without any validation with specific reporters. If our large-scale data are unable to #predict what happens in an artificially-imagined situation they are useless.

#sequencing #large-scale #sciencerant #research

2 prejudices I had on HMW extraction for long read sequencing:

I thought column kits were no good for HMW DNA, but latest Zymo miniprep(!) kits can do 15kb ~ >50kb

Apparently there are reliable pulse gel tools out there for resolving HMW bands, like FemtoPulse. Here's our miniprep at 14kb fragment size:

#microbiology #sequencing #longread

FemtoPulse output showing 14kb band cluster to be used for downstream long read sequencing application

Every now and then, I look at trackers and then I come to my senses and then go nope.

More power to you if you like your vibrato values in hex but very, very nope.

I mean, they're a step forward from the MC4 and 8 but that's about it.

#DAWs #Sequencing #CVGate #MIDI

Pathogens often find their way into #wastewater & can be identified & quantified there by DNA #sequencing (widely known since COVID). Now research shows that this can be used as early warning system for outbreaks. https://www.theguardian.com/science/2025/mar/05/wastewater-disease-flu-outbreaks