#MinION

2025-11-04

#Bioinformatics Q:

I am working on my first #Minion metabarcoding run. I've got a library of dual-indexed amplicons (ITS2). Ran it through the Minion, base called, and now I'm working on demultiplexing.

I have discovered that some of my reads contain multiple amplicons. i.e., a single line in the fastq file includes index1-primer-read1-primer-index2-index3-primer-read2-primer-index4

Is that a problem with my library, the sequencing process, or is it something I can fix at this stage (i.e., computationally)?

2025-10-10

Respect to whoever went the extra mile to make this Minion letterbox.

I stopped to photograph it at the entrance to a rural driveway in Lyttelton Harbour. I like how yellow reflectors have been added to it can be seen by car traffic.

Some skilled parent must be very popular with their children. 😀

#letterbox #Minion #DespicableMe #GovernorsBay #LytteltonHarbour #NZ #Christchurch #MetalWork

A photo of a metal lettbox shaped like a Minion from the movie Despicable Me. On the right side it's a regular yellow minion, and on the left side it's an evil purple minion.

My full-resolution photo is on Flickr at https://www.flickr.com/photos/mollivan_jon/54834359776/
Dandyliondreamer (coms closed)dandyliondreamer.bsky.social@bsky.brid.gy
2025-08-27

spamton minion is real and he can hurt you #artwip #wip #spamton #deltarune #minion

spamton from deltarune as a minon 

this is a cry for help
j43147 :pentagram:j43147
2025-08-13

Oh I'm one of those, it's called operation often now??? This is Lunarbase Dagon facility shit... It's the Andromedan virus creation is fighting against... the virus vs. all creation.
facebook.com/share/r/1FAQPa8F9

2025-08-13

@nanopore DRS sequencing temperature was lowered to 30/31C (#P2 / #MinION) in #minknow v24.06 (and all recent versions) slowing down RNA helicase from 130 bps to 100-110 bps, lowering yield per hour and affecting signal readouts.
In effect, recent and older #RNA004 runs differ in base- and modification calling quality, possibly pA tail estimates.
This change was not widely announced to the community apart from a brief mention in software release notes!
community.nanoporetech.com/pos
#academicChatter

screenshot from nanopore community post mentioning new temparatures for DRS
Oliver Bildesheimbildesheim@d-64.social
2025-07-24

#minion gefunden

Mark Daniels-Wr. 🟢MarkDW@mstdn.social
2025-07-02

A most excellent #minion #pool #birthdayCake 🤓
(made by very talented daughter 👍)

2025-06-14

Neue Creation
gehäkelter Eierwärmer Minons
#häkeln #basteln #minion #handmade

Leonardo Vieira Borgesmastero_schnedder
2025-05-27

Eu quando o aplicativo me bloqueia após eu mudar minha foto de perfil e nome de usuário:

FanCityKnits 🇺🇦🧶FanCityKnits@troet.cafe
2025-05-07

It's a #minion ! No, it's a #gnome !

Whatever - it's cute.

A #crochet pattern by #designer Oksana Kovalenko from #Ukraine

Minioon Gnome

ravelry.com/patterns/library/m

@crochet @fiberarts
#StandWithUkraine
#PlushToys
#handmade

Sound effect of the day: "snirk!" #lowbrowart #drawing #minion #cartoon #mutant
2025-04-18

Omg this 🙏🙏🙏

#meme #memes #minion #pronoun

Minion meme. Kids today just want to hear their pronouns but my generation wanted to hear “i now pronouns you man and wife”
Zougla.grzougla
2025-04-10

ZARA: Νέο κατάστημα στο ιστορικό κτίριο του Μινιόν στην Αθήνα zougla.gr/money/etairika-nea/z

Peter Rileypeterjriley2024
2025-03-09

Australia's Prime , Anthony Albanese (aka ) 2023 historic $368 billion deal with for eight USA nuclear-powered submarines in “three decade” … hard to swallow crunchy sub.

streetartcities.com/markers/69


2025-01-18
It's the hot chocolate festival in Vancouver, BC. This is a banana hot chocolate at Chez Christophe.

I was worried this would taste like a banana candy or like the banana medicine I had as a kid. Instead it tastes like chocolate chip banana bread with an apple caramel drizzle. So good! If this was a year round option, I would order it regularly.

#hotChocolateFest #WhiteRockBC #BurnabyBC #VancouverBC #Vancouver #Minion
A white wide mug with a light yellow whip. There is a golden drizzle over the whip. On the white saucer below the mug there is a yellow macaron with a flat white chocolate on top. The macaron top has thin chocolate lines to make it look like an eye, mimicking the appearance of a minion from Despicable Me.
Kevin Karhan :verified:kkarhan@infosec.space
2025-01-18

@TonyStark Considering the fact that #Musk allegedly did the biggest non-nuclear explosion and constantly runs his businesses into the ground, it's safe to say every Kindergartener is more competent because unlike #ApartheidEmeraldBoy they'd let adults deal with the important bits...

Musk thinks he's "#TonyStark" when in fact he's not even a #BondVillain #minion!

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