I'll be giving an SBGrid webinar next week:

TITAN: Getting the most from NMR titration experiments with two-dimensional lineshape analysis

10 January, 12pm EST/5pm UK/6pm CET

More info and registration: https://sbgrid.org/webinars/

@nmrchat @structbio

Year end reminder to pause those spinning wheels more often (and watch ice crack in the harbor 🧊)

@sararah ♥️ perfect use of magnets > stickers #NMRchat #dei

My magnets from Working Towards Inclusivity in #Chemistry - Toronto arrived today!!! They are sooo cute.

Degrader Review Roundup 2. For those seeking a comprehensive overview of all the various modes of TPD, plus their application to oncology, this is a good one.
https://www.mdpi.com/1422-0067/23/23/15440

In about 2003 we bought a heavily used #Concept2 #rowing machine from a community gym for, I think, $150. It is a Model B that was manufactured in 1992.

Concept2 STILL provides COMPLETE #SUPPORT AND REPLACEMENT #PARTS for a 30 year old rowing machine. They still sell upgrades to the electronics. They have never end-of-lifed it. They also provide complete support for even older Model A machines.

I have almost total confidence that that will not be the case for your #Peloton, etc.

Nice and concise #review about the different #methods to increase effective molecular weight of #proteins <100 ​kDa through target protein binding or target protein fusion (#nanobody and symmetric #scaffolds) enabling structure determination with #cryoEM @strucbio

https://www.sciencedirect.com/science/article/pii/S2665928X22000290

RT @DanNomura@twitter.com

Excited to showcase our latest work by postdoc @NafsikaForte@twitter.com @UCB_Chemistry@twitter.com @berkeleyMCB@twitter.com & @NovartisScience@twitter.com on covalently recruiting E2 ubiquitin conjugating enzyme UBE2D for targeted protein degradation applications!! Also thanks to @TheMarkFdn@twitter.com! https://www.biorxiv.org/content/10.1101/2022.12.19.520812v1

🐦🔗: https://twitter.com/DanNomura/status/1605188206021865472

The Genome Sciences Department (https://www.gs.washington.edu) at UW is seeking candidates for the department chair. Our department is known for research in the areas of model organism and human genomics, #computationalbiology, and #genomics and #proteomics #techdev. Please boost ... https://apply.interfolio.com/114030

Final issue of our 40th #anniversary year!

Featured:
#LUBAC linear #ubiquitin signaling on #mitochondria
#Calnexin’s sugar-free taste in #lipid #bilayer
#Mitophagy receptors doubling in #pexophagy
#Cadherin-mediated #adhesion control (on the cover by Liang, Buckley et al)

...and more:
https://www.embopress.org/toc/14602075/2022/41/24

Introduction
My name is Chris and I am the Glycomics Core Director at Beth Israel Deaconess Medical Center/Harvard Medical School, Boston Massachusetts. I use mass spectrometry as the primary detection method for released glycans and glycoconjugates. There is still a lot to learn in the glycosciences so an unbiased technique like mass spec is my favorite tool. #TeamMassSpec #Glycotime

David Bakers talk
"The coming of age of de novo protein design" at the NIH from Dec. 14th

@strucbio

https://videocast.nih.gov/watch=46050

#proteindesign #enzymeengineering

Please share! We are recruiting a #postdoc to work on an exciting #TPD project combining #proteomics and #cancer biology, come join our team! https://jobrxiv.org/job/university-of-california-san-diego-27778-postdoc-in-cancer-chemical-biology-pharmacology/

I am very pleased to be hosting our final TPD seminar for 2022!
Join us on December 15th to hear about Arvinas' PROTAC Discovery Engine as told by @miklosbekes of @ArvinasInc

Musk’s anti-Fauci tweet is my breaking point; leaving the other app behind so might as well do an #introduction!

I’m an Associate Professor of #Immunology at the #UniversityofWashington in Seattle. My lab studies programmed cell death in the context of cancer, infection and autoimmunity. Check out OberstLab.org for more info.

Outside the lab, I spend as much time as I can outdoors: Biking, rock climbing, hiking and #birding with my wife and our Irish Water Spaniel Rufus. What a good boy!

A user’s guide for those new to heterobifunctional degraders and seeking some initial guidance on design, preparation, and characterization. Via the lab of @fleur Ferguson.
https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpz1.611

Hey Structural Biologists :protein:

#crystallography #MX #CryoEM #CryoET #NMR #SAXS #HDX #Alphafold #PDB #EMDB #StructuralBiology

I have created a group @strucbio .

If you tag this account in any relevant toot, it will automatically boost it.

So if you follow this account, any toots about structural biology should end up in your TL. The more people use it, the better it will be.

Boosting appreciated. :protein:

#InDels reshape #coenzyme specificity in #Rossmann #fold #enzymes from NAD-binding to SAM-binding

https://www.pnas.org/doi/10.1073/pnas.2207965119

#proteinengineering #proteinevolution #catalysis #moleculardynamics #xraycrystallography #PNAS

Here is one of my favorite papers this year, on how the ribosome holds the growing protein nascent chain near the peptidyl transferase center (PTC). https://www.nature.com/articles/s41557-022-01073-1
Not only great science but great people behind the science. #Ribosome #StructuralBiology

I am interested in using #NMR to understand biology, so my work varies from #biophysics #kinetics #dynamics #proteinfolding to #structuralbiology

An example of our collaborations, with @ak947 and @lapassmore where we showed that Fip1 dynamics are required for CPF activation. NMR was used to study the conformational dynamics of Fip1 within the 850-kDa CPF complex.

http://www.genesdev.org/cgi/doi/10.1101/gad.348671.121

#NMRchat #protein #IDP #biochemistry #mRNA #3'endprocessing