Lmst

Wang et al. report that CLE19 suppresses brassinosteroid signaling output via the BSL-BIN2 module to maintain BES1 activity and #pollen exine patterning in #Arabidopsis.

🌺https://doi.org/10.1111/jipb.70024

@wileyplantsci
#PlantScience #plant #reproduction #hormone #OpenAccess #botany

Yin/yang-styled diagram, illustrating that antagonistic crosstalk between the microspore-derived CLE19 peptide and brassinosteroid signaling preserves pollen developmental homeostasis in Arabidopsis. CLE19 activates a protein phosphatase—kinase cascade to phosphorylate the transcription factor BES1, triggering its inactivation and suppressing brassinosteroid signaling outputs, thereby fine-tuning male fertility under fluctuating conditions.

A mystery 30+ years in the making!

In this #OpenAccess Brief Report, Wu et al. describe a novel physiological function of the #Arabidopsis
PPRD1 in #brassinosteroid #biosynthesis, and new possibilities for alternative #steroid reductases involved in steroid #metabolism.

🌱 https://doi.org/10.1111/jipb.70022

#PlantSci

Panel of morphological and statistical data to illustrate findings that overexpression of Arabidopsis POLYPRENOL REDUCTASE 1 (PPRD1) partially rescues the de-etiolated2-1 (det2-1) brassinosteroid biosynthesis mutant phenotype and increases its brassinosteroid contents. A loss-of-function pprd1 mutation enhances the det2-1 short-root phenotype and further reduces its brassinosteroid levels, suggesting that PPRD1 plays a role in brassinosteroid biosynthesis.

💡Lee et al. demonstrate that the bZIP3 #transcription factor is important for CO-induced FT expression under long #photoperiod conditions in #Arabidopsis.

📑Read this #OpenAccess paper-and more-here!

➡️https://doi.org/10.1111/jipb.70014

🔗
@wileyplantsci
#PlantScience #JIPB #flowering #botany

A hypothetical model explaining how the bZIP3–AS1–CO complex regulates the temporal transcriptional regulation of FT. (A) Schematic representation of FT transcriptional regulation by bZIP3, AS1, and CO proteins. (B) Temporal regulation of FT expression by the function of bZIP3-AS1-CO complex in a photoperiod-dependent manner.

The histone variant H3.14 is an early player in the abiotic stress response of Arabidopsis.

#Epigenetics #HistoneMarks #HistoneVariants #AbioticStress #Arabidopsis #PlantScience

https://www.sciencedirect.com/science/article/pii/S1534580725003740

Plants repress ROS1 expression to attenuate heat-induced transposon burst.

#Transposons #Retrotransposons #ROS1 #RepressorOfSilencing1 #HeatStress #ONSEN #Arabidopsis #PlantScience

https://www.nature.com/articles/s41477-025-02076-9

T-DNA insertions: more surprises than you think 🌱🧬.
PCR can miss extra T-DNAs & complex rearrangements. Long-read sequencing captures the full picture. Still super relevant for plant genomics!
🔗 https://doi.org/10.1186/s12864-021-07877-8
#PlantScience #Genotyping #LongReadSequencing #Arabidopsis @PuckerLab

Genomic rearrangements in the A.thaliana T-DNA line GK-654A12. Source: https://doi.org/10.1186/s12864-021-07877-8

🌱Annals of #Botany invites submissions to the new Special Issue "Exploring seed development, structure and physiology", edited by AoB editors Gabriela Auge and Chunhui Zhang
This call for papers is associated with a concurrent session at the International Conference on #Arabidopsis Research 2025 and the 4th Argentinian Meeting in #SeedBiology
#seeds #plantscience #specialissue
‪@gabyplantbio.bsky.social‬
https://bsky.app/profile/annbot.bsky.social/post/3lv43dxq3ak24

Photoperiod sensitivity affects #flowering time and, ultimately, #yield. Wang et al. reveal new insights into how the LUX-SWI3C module regulates #photoperiod sensitivity in #Arabidopsis at the #epigenetic level.
https://doi.org/10.1111/jipb.13889
@wileyplantsci
#PlantSci #JIPB #botany

Proposed model for the LUX and SWI3C regulation of photoperiod sensitivity in LD WT, SD WT, and LD/SD lux swi3c mutant plants.

A comprehensive atlas of full-length Arabidopsis eccDNA populations identifies their genomic origins and epigenetic regulation.

#ExtrachromosomalCircularDNA #eccDNA #Transposons #Arabidopsis #MethylationMutants #LongReadSequencing

https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3003275

Long-read detection of transposable element mobilization in the soma of hypomethylated Arabidopsis thaliana individuals.

#Transposons #SomaticTransposition #Hypomethylation #MET1 #Arabidopsis #LongReadSequencing

https://link.springer.com/article/10.1186/s13059-025-03691-7

More #free #PlantScience? We thought you'd never ask!
Get a sneak peek at this remarkable work by Zhang et al. that reveals how scaffold #protein RACK1 regulates #root #growth and gravitropic response.
🔓👇
https://doi.org/10.1111/jipb.13858
@wileyplantsci
#JIPB #auxin #Arabidopsis #botany

Diagram representing a cross-section through the plasma membrane and cytosol to illustrate how the scaffolding protein RACK1 is involved in polar auxin transport and signaling, binding to PINOID and PIN-FORMED2, enhancing their interaction and phosphorylation-dependent auxin efflux. Knocking down RACK1 genes impairs auxin-related processes such as root growth and gravitropic response.

Previous studies of extrachromosomal circular DNA #eccDNA in #plants are based on short-read seq. @vanderschuren-lab.bsky.social uses long-read seq to obtain a map of full-length eccDNAs from #Arabidopsis, identifying their genomic origins & epigenetic regulation @PLOSBiology plos.io/44Mq3RI

Dynamics and distribution of eccDNA in Arabidopsis. Left: Schematic representation of eccDNA amplification and sequencing via CIDER-Seq pipeline. Top right: EccDNA size distribution in Arabidopsis Col-0 leaf tissues (n = 4,158). The x-axis shows eccDNA size in kb and the y-axis shows the percentage of eccDNAs belonging to each window. Middle right: Size ranges of eccDNAs with marked median and quantiles (n = 4,190). Bottom right: Percentages of eccDNA reads mapping to TAIR CDS, TE, or intergenic databases (values represent the average of three replicates).

A plant's #microbiome can protect it against detrimental bacterial strains. This study shows that competition among #Pseudomonas #bacteria in synthetic bacterial communities determines how well they can protect #Arabidopsis; a single competitive strain can suffice @PLOSBiology https://plos.io/4o96mN1

Arabidopsis thaliana Col-0 phenotypes grown on MS agar plates alone (MS) or in co-cultivation with the indicated SynComs (AtSC5, AtSC6, LjSC5, LjSC6), and after treatment with mock (Control), R401 wild-type (R401wt) or R401 ΔpvdY ΔphlD (R401mut) strain. Scale bar corresponds to 1 cm.

In the greenhouse, #Arabidopsis with hyperactive ACD6 have stronger pathogen defenses but are smaller & make fewer seeds. @derekseveri @plantevolution &co show that in the open field both advantages & disadvantages of the allele disappear @PLOSBiology https://plos.io/465XPDR

A hyperactive ACD6 allele reduces growth and causes necrosis only in the greenhouse. Top: Representative Arabidopsis thaliana rosettes from different genetic backgrounds. Yellow and blue labels represent descendants of a heterozygous ACD6 HIF line that are homozygous for either the Est-1 or the Col-0 allele of ACD6. Green and purple labels represent the Est-1 accession and the isogenic acd6 knockout null allele in that accession. Plants were grown in Tübingen in the greenhouse (GH-Tü), outdoors in the field in Tübingen (F-Tü), or outdoors in the field in Zurich (F-Z, a GMO-approved facility). Note the necrotic older leaves for greenhouse plants with the ACD6-Est-1 allele (red arrows). Bottom: Rosette size of individual plants, calculated by quantifying green pixels from images similar to those in the top panel. The line within each box represents the median. Boxes enclose the interquartile range (IQR) with whiskers extending to up to 1.5 times the IQR. Brackets indicate Mann–Whitney U-test results, with * indicating P < 0.05 after Benjamini–Hochberg correction for multiple testing across the four comparisons. Dotted lines extending from the mean of each distribution span the observed effect size, and a bootstrapped distribution of computed effect sizes [30] is shown in gray, centered on the mean of the second box in each pair (red dot). The black bar extending vertically from the red dot represents the 95% confidence interval of the effect size.

And it gets a bonus point for discussing its genome and referencing The Arabidopsis Information Resource (TAIR).

#ArabidopsisThaliana #Arabidopsis

New work by Gong et al. on FGM1/rPPR4 in #Arabidopsis reveals that essential mechanisms are established during female gamete maturation to provide a #molecular basis for #seed #development initiation and #plant #fertility.
https://doi.org/10.1111/jipb.13922
@wileyplantsci
#PlantSci #JIPB #botany

Egg cell and endosperm GFP micrographs of WT and mutant plants illustrate that FEMALE GAMETE MATURATION 1/PENTATRICOPEPTIDE REPEAT PROTEIN 4), a component of the mitochondrial ribosome large subunit, directly interacts with another mitochondrial protein GAMETE CELL DEFECTIVE 1 to play an essential role in maturation of the egg cell and central cell.

The FLC gene helps determine #FloweringTime variation in #Arabidopsis. Analysis of FLC mutants across 62 natural #genetic backgrounds shows how pan-genetic analysis of hub genes can reveal the extent of their broader genetic network @PLOSBiology https://plos.io/3ZkZNvV

Left: An early-flowering plant in the wild (at the Angit site in Southern Italy. Right: Flowering time of progeny from wild plants and control genotypes in the greenhouse (22°C long days).

Organisms have evolved complex and sophisticated #DNA damage response (DDR) mechanisms. In a new #JIPB Brief Comms feature, Yang et al. report on their efforts to identify new DDR regulators in plants
https://doi.org/10.1111/jipb.13910
@wileyplantsci
#PlantSci #Arabidopsis #clathrin #botany #endocytosis

This panel of images illustrates data supporting the report that Clathrin is required for DNA damage repair. Clockwise from upper left: Morphology and relative root lengths of sampled seedlings, GUS staining images of cotyledons, data representing relative HR efficiency, and confocal imaging of subcellular localization of CHC1 and CLC2.

New paper in MBE by Pfalz et al - genetic variation at a near-cryptic QTL for defense metabolites in Arabidopsis is shaped by balancing selection and ectopic gene conversion among duplicated O-methyltransferase genes.

🔗 https://doi.org/10.1093/molbev/msaf086

#evobio #molbio #arabidopsis #crisprcas9

This new #JIPB paper by Xu et al. illustrates the role of the SPL10–REV module in orchestrating #leaf curvature and the potential use in #molecular #breeding of #economically important traits in #vegetable #crops.
https://doi.org/10.1111/jipb.13893
@wileyplantsci
#PlantSci #Arabidopsis #Botany

#Arabidopsis

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