For #fluorescencefriday I just thought these neurons in the lateral geniculate nucleus were pretty. #sciart #microscopy #confocal

Escurinho do confocal

—
URL: https://brunovellutini.com/posts/escurinho-do-confocal/

#confocal #microscopia

A new study introduces two yellow #fluorescent proteins—mGold2s and mGold2t—that are significantly more #photostable than commonly used alternatives such as mVenus and mCitrine. Using these new #YFPs, researchers were able to extend imaging durations across diverse modalities including widefield, total internal reflection fluorescence (#TIRF), super-resolution, single-molecule, and laser-scanning #confocal microscopy. Learn more: https://ilphotonics.com/introducing-two-new-yellow-fluorescent-proteins/

#MSLASpheroidStamp: #OpenSource 3D #cell #spheroids for everyone:

-#3Dprinted molds/stamps
-time-lapse #confocal #microscopy of spheroids
-#cytotoxicity screens

Preprint: https://doi.org/10.1101/2024.05.12.593682
GitHub: https://github.com/arteys/MSLASpheroidStamp
#DIYbio #lab #instruments #MSLA #3Dprinting #bioprinting #imaging

A research team developed a budget-friendly method for adapting confocal microscopes for Total Internal Reflection Fluorescence (#TIRF) microscopy. The paper presents “a simple and cost-effective method to build a #prism-based TIRF setup on a laser scanning confocal microscope. The setup is affordable, and users can use both #confocal and TIRF modes in the same setup with the same light source and optical components.” Learn more: https://ilphotonics.com/inexpensive-high-resolution-single-molecule-microscopy/

Drain fly embryo on Nikon Small World

Nikon Small World has announced the winners of this year’s photomicrography competition. My image of a drain fly embryo made it to the Images of Distinction category!

—
URL: https://brunovellutini.com/posts/drain-fly-embryo-nikon-small-world/

#clogmiaAlbipunctata #confocal #diptera #embryo #image #microscopy #molecular #scienceOutreach

Interesting new #preprint about #tissueclearing and #lightsheet #microscopy in mouse ovaries. Complete with #napari-based deep-learning pipeline and detailed build instructions for 3D-printed sample chambers for solvent-cleared samples, so they can be viewed under a #confocal!

OoCount: A machine-learning based approach to mouse ovarian follicle counting and classification
Folts et al., preprint at biorxiv 2024
https://www.biorxiv.org/content/10.1101/2024.05.13.593993v1

Confocal excitation

The pipetting marathon I went through the other day was worth it—the experiment worked! Now I get to image some exciting and colorful samples in the confocal microscope, and experience that thrill of seeing something for the first time, once again 🌈🔬

—
URL: https://brunovellutini.com/posts/confocal-excitation/

#confocal #germany #microscopy #molecular #postdoc

Ready for the new year: new #NSPARC detector for image scanning #microscopy is installed on the #Nikon #AX MP #multiphoton #microscope @ #MIC in #Muenster. Waiting for high-resolution multiphoton images.

#ISM
#2-Photon
#confocal

Also including an interesting discussion about the feasibility of measuring photostability at all in a point-scanning system, beginning with the following curious sentence:

"Shaner et al., who belonged to the laboratory of the late Dr. Roger Tsien, pioneered and successfully standardized the quantification of photostability in the field of FP technology. "

#GFP #fluorescence #confocal #microscopy

Hello everyone,
Just moved my profile to this server - this is a good chance for a late introduction.
Interested in microscopy, bioimaging, confocal, superresolution microscopy, molecular motors and biological topics in general.

#microscopy #confocal #superresolution #molecularmotors #ruhrpott #introduction

Cardiomyocyte orientation recovery at micrometer scale reveals long-axis fiber continuum in heart walls

A new method that combines #confocal #microscopy with computer vision provides unprecedented spatial resolution of #cardiomyocyte geometry along the mouse heart wall.

Minhajuddin Sirajuddin, Kaleem Siddiqi et al

https://www.embopress.org/doi/full/10.15252/embj.2022113288

#throwbackthursday Here is an old image I took many moons ago.
#confocal #microscopy image of #MouseFibroblast cell with multiple nuclei. False coloured and inverted to be on a white background. Blue microtubules, green nuclei

RT: @svi_huygens@twitter.com

#microscopists, want to get a better overview of microscopy image #FileFormats, bit depth, scaling, image #metadata, dimensions, and more?

Join our webinar Feb 23 and explore what formats e.g. #OMETIFF:

https://svi.nl/WebinarInvitation

#microscopy #ImageAnalysis #confocal

🔶 What are the additional insights you can gain from imaging tissue sample in 10 colors?
🔶 Follow our on-demand webinar with Dr. Nicolas Gaudenzio and discover how to perform 10-color imaging using a confocal microscope. See how this allows to assess the skin immune landscape.

https://fcld.ly/lciceh8

#STED #STELLARIS #confocal #immune

Jan-Lukas Førde et al. present a new #software tool for automatic #cell segmentation of fluorescent #cancer cells in #zebrafish larvae:

https://journals.biologists.com/bio/article/11/12/bio059530/285931/A-new-software-tool-for-computer-assisted-in-vivo

#Imaging #Biology #BiologyOpen #Science #AcademicMastodon #Confocal #Segmentation

The new #nikon #NSPARC #AX #Confocal #microscope uses #ism image scanning microscopy and a spatial array detector to enable high sensitive #superresolution microscopy with a point scanning confocal.

https://www.microscope.healthcare.nikon.com/en_EU/products/confocal-microscopes/ax/ax-ax-r-with-nsparc

Tim Salditt (http://twitter.com/SaldittLab, substituting Jasper Frohn): #Multiscale #Xray Phase Contrast #Tomography at #GINIX/P10: Concepts, Implementation and Applications

- fantastic collaboration with the P10 team
- directly comparing #STED to #Minflux; old #confocal? not worth mentioning 😂
- overview tomo, then zoom-in
- tumorous human pancreatic tissue #biopsy: quantify the #tumor type
- from electron density to metrics, quantify fibres etc.
- #hippocampus patho punch, #Alzheimer sample

Pui-Ying (Penny) Lam imaged adult #Danionella cerebrum in vivo for the first time using standard #confocal microscopy in 2022!

https://journals.biologists.com/dmm/article/15/12/dmm049753/286133/Longitudinal-in-vivo-imaging-of-adult-Danionella