#barcoding

Frédéric Rimetrimetfrederic
2025-11-27

and in supp data the logo of diat.barcode ;-)

Frédéric Rimetrimetfrederic
2025-11-27

Happy to share our new paper presenting a new version of diat.barcode reference for
Gelis, Barry-Martinet, Briand, Chonova, Cochero, Gassiole, Kahlert, Karthick, Keck, Kelly, Kochoska, Mann, Nayak, Pfannkuchen, Servulo, Trobajo, Vasselon, Vidakovic, Viollaz, Wetzel, Zimmermann, Rimet, F. (2025). doi.org/10.1051/limn/2025009

Taxonomic tree of Diat.barcode v12. Hierarchical relationships of families according to their parent taxonomical links are represented. Size of bubbles gives the number of sequences in the corresponding families. Colors correspond to Classes: purple: Mediophyceae, red: Bacillariophyceae, green: Coscinodiscophyceae, blue: Fragilariophyceae.Ecological guild distribution in the phylogeny (based on rbcl). Black color corresponds to a sequence belonging to an ecological guild. From inner to outer circle: high-profile, low profile, motile, planktonic.Ordination biplots for Mainland France obtained from symmetric co-correspondence analysis (sCoCA). Ecological guilds are displayed in the left panel and species in the right panel. To improve the interpretation of the plots, species are colored based on their family and polygons represent the sampling sites from which they were sampled, regardless of their identification.Navicula photo F Rimet
Cytology and Geneticscytgen
2025-05-22
2025-03-04

Die Frage "Was krabbelt denn hier?" ist bei #Spinnen nicht leicht zu beantworten. Yannis Schoeneberg von der Trier möchte samt Kollegen per Multi-Locus-#Barcoding das „taxonomische Rennen“ gewinnen, bevor es zu spät ist … Mehr dazu von Karin Lauschke: laborjournal.de/editorials/319

Foto einer Ryuthela-Gliederspinne. Darin oben rechts als rundes Inset: ein Porträtfoto des Trierer Spinnenforschers Yannis Schöneberg. rechts neben dem Foto Überschrift "Was krabbelt denn da?" des im Post verlinkten Arikels, gefolgt von dessen Vorspann.
2025-01-21

#dnaderived #sequences #barcoding #Cortinarius Added another several dozens of sequences to our Fungarium YSU database, which soon will be discoverable though GBIF. Hope to find collaboration through shared barcoding initiatives. Especially like these days Cortinarius spp., they are amazing.
Totally about 1K specimens in our collection have dna-derived data (attached sequences): gbif.org/dataset/d922b606-6c94

Frédéric Rimetrimetfrederic
2024-05-16

Nice presentation of Adriana Radulovici on @leeselab
20 years after Paul Hebert paper on DNA who could expect this wordwide success?

Frédéric Rimetrimetfrederic
2024-04-18

Nice presentation of carbon footprint of molecular methods by O Monnier and his student Mathieu.

Frédéric Rimetrimetfrederic
2024-03-27

Excellent paper from F Keck.

Reference barcoding libraries problems:
(i) mislabelling, (ii) sequencing errors, (iii) sequence conflict, (iv) taxonomic conflict, (v) low taxonomic resolution, (vi) missing taxa and (vii) missing intraspecific variants. They give possible consequences on the taxonomic assignment process

Navigating the seven challenges of taxonomic reference databases in metabarcoding analyses
doi.org/10.1111/1755-0998.13746

2023-11-17

RT by @Plants_EFSA: Behind the scenes, indentify #2 : @aimethods DNA Barcoding.

Black-headed leafcutter bee, Megachile circumcincta. The DNA was isolated from a leg of the bee using a liquid (lysis buffer). Means everything dissolves in it except for the chitin. Then the Co1 gene was amplified with PCR. This means that certain parts of the DNA are copied so often that they are easily detectable. Detectable for the sequencer, the next step. The 313 base pairs required for the identification are then determined here. (See colorful graphic) With the help of a worldwide database of already certain animals whose Co1 gene has been determined, the base pairs can be compared with each other.

#dnabarcoding #barcoding #dna #macro #insects #bumblebee #hummel #insekten

🐦🔗: nitter.cz/sagaOptics/status/17

[2023-11-16 16:01 UTC]

Frédéric Rimetrimetfrederic
2023-11-11

Nice courses that we gave to students in University of Torino with Kalman Tapolczai on diatom metabarcoding. Thank you to Francesca Bona and Elisa Falasco for the invitation
@Kalipster

2023-10-23

Just published: "Marine invasive alien species in Europe: 9 years after the #IAS Regulation"

Proud to contribute to the #eDNA part of this review on #BioMonitoring of #marine #invasive #alien species in Europe, as part of the #GES4SEAS project. Have a look, esp. if you wondered how many #NIS species for which sequence vouchers exist for #DNA #barcoding or #metabarcoding

frontiersin.org/articles/10.33

2023-10-13

#biodiversity #genomics #training 🧑‍🏫with BGE partners perfecting techniques for HMW #DNA 🧬 extractions for #genome assembly & plates of #arthropods 🐞 for #barcoding hosted by Auth University Thessaloniki 🇬🇷
@erga_biodiv @SIB

Frédéric Rimetrimetfrederic
2023-09-27

Here is our new paper about diatom metabarcoding :😋
Filling reference libraries with diatom environmental sequences: strengths and weaknesses

tandfonline.com/doi/full/10.10

2023-09-20

Here is a free read-only link to our latest paper on retention and export of planktonic fish eggs in the northeastern Gulf of Mexico #fish #barcoding #DNA #oceanography #science #USFCMS #fisheries
onlinelibrary.wiley.com/share/

Frédéric Rimetrimetfrederic
2023-09-18

Very proud of our new paper:😀

Canino, A., C. Lemonnier, B. Alric, A. Bouchez, I. Domaizon, C. Laplace-Treyture, & F. Rimet, 2023. Which barcode to decipher freshwater microalgal assemblages? Tests on mock communities. International Journal of Limnology EDP Sciences 59: 8, 1-15. DOI: 10.1051/limn/2023008

limnology-journal.org/articles

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